Prolonged systemic inflammation and damage to the vascular endothelium following intratracheal instillation of air pollution nanoparticles in rats

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Author
Adams, Rachel
Potter, Stephen
Berube, Kelly
Jones, Tim
Evans, Shelley-Ann
Date
2018-12-21Acceptance date
2018-11-30
Type
Article
Publisher
IOS press
ISSN
1386-0291
1875-8622 (online)
Metadata
Show full item recordAbstract
BACKGROUND: Exposure to air pollution is associated with cardiovascular disease, including increased morbidity and mortality rates.
OBJECTIVE: The aim of this investigation was to assess the effect, in rats, of intratracheal instillation of particulate air pollution on biomarkers of leucocyte activation and vascular endothelial damage.
METHODS: Air pollution particles (PM10) were instilled into rats, and blood samples were taken three days and six weeks post instillation. Plasma neutrophil elastase and VonWillebrand factor were measured by ELISA.
RESULTS: Plasma neutrophil elastase increased from 175±44 ng/ml at baseline to 288±26 ng/ml 3 days post instillation (p=0.038). vWF increased from 0.160±0.015 IU/ml at baseline to 0.224±0.015 IU/ml at 3 days post and 0.208±0.01 IU/ml at 6 weeks post (p=0.006, ANOVA). sICAM-1 increased from 17.75±0.70 ng/ml at baseline to 19.03±0.33 ng/ml at 3 days post and 21.72±1.16 ng/ml at 6 weeks post (p=0.009, ANOVA).
CONCLUSION: Instillation caused prolonged systemic inflammation, activation of blood leucocytes and damage to the vascular endothelium.
Journal/conference proceeding
Clinical Hemorheology and Microcirculation;
Citation
Adams, R.A., Potter, S., Bérubé, K., Higgins, T.P., Jones, T., and Evans, S-A. (2018) 'Prolonged systemic inflammation and damage to the vascular endothelium following intratracheal instillation of air pollution nanoparticles in rats', Clinical Hemorheology and Microcirculation. https://dx.doi.org/10.3233/CH-180377
Description
Article published in Clinical Hemorheology and Microcirculation, available at https://dx.doi.org/10.3233/CH-180377
Sponsorship
Cardiff Metropolitan University (Grant ID: Cardiff Metropolian (Internal))
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