Protection of the Ovine Fetal Gut against Ureaplasma-Induced Chorioamnionitis: A Potential Role for Plant Sterols
van Gorp, Charlotte
de Lange, I.H.
Cillero Pastor, B.
van Gemert, W.L.
Beeton, Michael L.
MetadataShow full item record
Chorioamnionitis, clinically most frequently associated with Ureaplasma, is linked to intestinal inflammation and subsequent gut injury. No treatment is available to prevent chorioamnionitis-driven adverse intestinal outcomes. Evidence is increasing that plant sterols possess immune-modulatory properties. Therefore, we investigated the potential therapeutic effects of plant sterols in lambs intra-amniotically (IA) exposed to Ureaplasma. Fetal lambs were IA exposed to Ureaplasma parvum (U. parvum, UP) for six days from 127 d–133 d of gestational age (GA). The plant sterols β-sitosterol and campesterol, dissolved with β-cyclodextrin (carrier), were given IA every two days from 122 d–131 d GA. Fetal circulatory cytokine levels, gut inflammation, intestinal injury, enterocyte maturation, and mucosal phospholipid and bile acid profiles were measured at 133 d GA (term 150 d). IA plant sterol administration blocked a fetal inflammatory response syndrome. Plant sterols reduced intestinal accumulation of proinflammatory phospholipids and tended to prevent mucosal myeloperoxidase-positive (MPO) cell influx, indicating an inhibition of gut inflammation. IA administration of plant sterols and carrier diminished intestinal mucosal damage, stimulated maturation of the immature epithelium, and partially prevented U. parvum-driven reduction of mucosal bile acids. In conclusion, we show that β-sitosterol and campesterol administration protected the fetus against adverse gut outcomes following UP-driven chorioamnionitis by preventing intestinal and systemic inflammation.
van Gorp, C., de Lange, I.H., Spiller, O.B., Dewez, F., Cillero Pastor, B., Heeren, R., Kessels, L., Kloosterboer, N., van Gemert, W.G., Beeton, M.L., Stock, S.J., et al. (2019) 'Protection of the Ovine Fetal Gut against Ureaplasma-Induced Chorioamnionitis: A Potential Role for Plant Sterols', Nutrients, 11(5), p.968.
Article published in Nutrients, available open access at https://doi.org/10.3390/nu11050968
Cardiff Metropolitan University (Grant ID: Cardiff Metropolian (Internal))
Showing items related by title, author, subject and abstract.
Cross-sectional study on the nutritional awareness of plant-based diets and associated attitudes among vegans, vegetarians and omnivores in South Wales Garbutt, James Daniel (Cardiff Metropolitan University, 2017-06-01)Background - There is currently an incomplete picture of nutritional awareness of plant-based diets within the UK. Without an understanding of this nutritional awareness amongst different dietary groups, it will be difficult ...
The antimicrobial activity of Sudanese honeys alone and in combination with plant extracts and ethylenediamineteraacetic acid (edta) Hashim, Ahmed I. (Cardiff Metropolitan University, 2015)Honey and plant extracts have a long hitory of medicinal use in Sudan that continues to the present day. The antimicrobial properties of Manuka honey and its use as a topical wound treatment is now widely recognised but ...
Use of NDVI (Normalized Difference Vegetation Index) and Precision Agriculture methodologies to evaluate field variability in important soil properties Kilatzidis, Savvas (Cardiff Metropolitan University, 2013)Corn (Zea mays L.) is one of the most important feed food and fuel crops today, worldwide. They have been extensive work on variety improvement through conventional plant breeding as well as using genetic engineering ...