Genome Analysis, Metabolic Potential, and Predatory Capabilities of Herpetosiphon llansteffanense sp. nov.
American Society for Microbiology
MetadataShow full item record
Herpetosiphon spp. are ubiquitous, chemoheterotrophic, filamentous gliding bacteria with the ability to prey on other microbes through a “wolf pack” mechanism. The genus currently comprises four known species (H. aurantiacus, H. geysericola, H. giganteus, and H. gulosus), which produce antimicrobial secondary metabolites such as siphonazole. As part of a study isolating myxobacterial wolf pack predators, we serendipitously isolated a novel environmental strain (CA052B) from the edge of a stream at Llansteffan, United Kingdom, which was identified as a member of the Herpetosiphon genus. A lawn culture method was utilized to analyze the predatory activity of CA052B against 10 prey organisms of clinical relevance. CA052B was found to prey on Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Enterococcus faecalis, Bacillus subtilis, and Candida albicans. Purified CA052B outer membrane vesicles also exhibited killing activity against the prey organisms when tested by flow cytometry. 16S rRNA sequencing of CA052B showed 98 to 99% identity with other Herpetosiphon species members. Comparing the genome of CA052B with the publicly available genomes of H. aurantiacus and H. geysericola revealed average nucleotide identities of only 84% and 91%, respectively, whereas the genome-to-genome distance calculation showed sequence identities of 28.2% and 46.6%, respectively. Biochemical characterization also revealed distinctions between CA052B and both H. gulosus and H. giganteus. Thus, strain CA052BT (= DSM 107618T = NBRC 113495T) is proposed to be the type strain of a novel species, Herpetosiphon llansteffanense sp. nov. The genome sequence of CA052B also revealed diverse secondary metabolite biosynthetic clusters, encouraging further exploration of its antibiotic production potential.
Applied and Environmental Microbiology;
Livingstone, P.G., Morphew, R.M., Cookson, A.R. and Whitworth, D.E. (2018) 'Genome Analysis, Metabolic Potential, and Predatory Capabilities of Herpetosiphon llansteffanense sp. nov.', Applied and Environmental Microbiology, 84(22), pp.e01040-18. DOI: 10.1128/AEM.01040-18.
Article published in Applied and Environmental Microbiology on 30 October 2018, available open access at: https://doi.org/10.1128/AEM.01040-18.
Cardiff Metropolitan University (Grant ID: Cardiff Metropolian (Internal))
Genome sequencing was provided by MicrobesNG, which is supported by the Biotechnology and Biological Sciences Research Council (BBSRC grant number BB/ L024209/1). The Institute of Biological Environmental and Rural Sciences receives strategic funding from the BBSRC.
Showing items related by title, author, subject and abstract.
Genome sequencing and pan-genome analysis of 23 Corallococcus spp. strains reveal unexpected diversity, with particular plasticity of predatory gene sets Livingstone, Paul; Morphew, Russell; Whitworth, David (Frontiers Media, 2018-12-19)Corallococcus is an abundant genus of predatory soil myxobacteria, containing two species, C. coralloides (for which a genome sequence is available) and C. exiguus. To investigate the genomic basis of predation, we ...
Laksari, Hafsa (Cardiff Metropolitan University, 2018-07)This research has been conducted to gain an insight into the world of computational tools that map genomes. Mapping genomes to the correct reference genomes, allows professionals to give an accurate diagnostic of any ...
Genome-Wide Identification of Myxobacterial Predation Genes and Demonstration of Formaldehyde Secretion as a Potentially Predation-Resistant Trait of Pseudomonas aeruginosa Sutton, Daryn; Livingstone, Paul; Furness, Eleanor; Swain, Martin; Whitworth, David (Frontiers Media, 2019-11-13)Despite widespread use in human biology, genome-wide association studies (GWAS) of bacteria are few and have, to date, focused primarily on pathogens. Myxobacteria are predatory microbes with large patchwork genomes, with ...