Campylobacter jejuni biofilms contain extracellular DNA and are sensitive to DNase I treatment
Brown, Helen Louise
Van Vliet, Arnoud
MetadataShow full item record
Biofilms make an important contribution to survival and transmission of bacterial pathogens in the food chain. The human pathogen Campylobacter jejuni is known to form biofilms in vitro in food chain-relevant conditions, but the exact roles and composition of the extracellular matrix are still not clear. Extracellular DNA has been found in many bacterial biofilms and can be a major component of the extracellular matrix. Here we show that extracellular DNA is also an important component of the C. jejuni biofilm when attached to stainless steel surfaces, in aerobic conditions and on conditioned surfaces. Degradation of extracellular DNA by exogenous addition of DNase I led to rapid biofilm removal, without loss of C. jejuni viability. Following treatment of a surface with DNase I, C. jejuni was unable to re-establish a biofilm population within 48 h. Similar results were obtained by digesting extracellular DNA with restriction enzymes, suggesting the need for high molecular weight DNA. Addition of C. jejuni genomic DNA containing an antibiotic resistance marker resulted in transfer of the antibiotic resistance marker to susceptible cells in the biofilm, presumably by natural transformation. Taken together, this suggest that eDNA is not only an important component of C. jejuni biofilms and subsequent food chain survival of C. jejuni, but may also contribute to the spread of antimicrobial resistance in C. jejuni. The degradation of extracellular DNA with enzymes such as DNase I is a rapid method to remove C. jejuni biofilms, and is likely to potentiate the activity of antimicrobial treatments and thus synergistically aid disinfection treatments.
Frontiers in Microbiology;
Brown, H.L., Hanman, K., Reuter, M., Betts, R.P. and Van Vliet, A.H. (2015) 'Campylobacter jejuni biofilms contain extracellular DNA and are sensitive to DNase I treatment', Frontiers in Microbiology, 6, p.699. DOI: 10.3389/fmicb.2015.00699.
Article published in Frontiers in Microbiology on 10 July 2015, available open access at: https://doi.org/10.3389/fmicb.2015.00699.
Cardiff Metropolitan University (Grant ID: Cardiff Metropolian (Internal))
Showing items related by title, author, subject and abstract.
Okhiria, Olusola Adeola (University of WalesCardiff School of Health Sciences, 2010)Biofilms have long been implicated in persistent infections and have recently been associated with chronic wounds. The role of bacteria in wounds is not yet fully understood and their ability to form biofilm is yet to be ...
Cooper, Rose; Bjarnsholt, T.; Alhede, M. (Europe PMC Plus, 2014)Following confirmation of the presence of biofilms in chronic wounds, the term biofilm became a buzzword within the wound healing community. For more than a century pathogens have been successfully isolated and identified ...
Irie, Yasuhiko; Roberts, Aled Edward Lloyd; Kragh, Kasper; Gordon, Vernita; Hutchison, Jaime; Allen, Rosalind; Melaugh, Gavin; Bjarnsholt, Thomas; West, Stuart; Diggle, Stephen (American Society for Microbiology, 2017-06-20)Extracellular polysaccharides are compounds secreted by microorganisms into the surrounding environment, and they are important for surface attachment and maintaining structural integrity within biofilms. The social nature ...