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dc.contributor.authorSutton, Daryn
dc.contributor.authorLivingstone, Paul
dc.contributor.authorFurness, Eleanor
dc.contributor.authorSwain, Martin
dc.contributor.authorWhitworth, David
dc.date.accessioned2020-01-15T10:30:43Z
dc.date.available2020-01-15T10:30:43Z
dc.date.issued2019-11-13
dc.identifier.citationSutton D., Livingstone P.G., Furness E., Swain M.T. and Whitworth D.E. (2019) 'Genome-Wide Identification of Myxobacterial Predation Genes and Demonstration of Formaldehyde Secretion as a Potentially Predation-Resistant Trait of Pseudomonas aeruginosa', Frontiers in Microbiology, 10(2650). DOI: 10.3389/fmicb.2019.02650.en_US
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10369/10890
dc.descriptionArticle published in Frontiers of Microbiology on 13 November 2019, available open access at: https://doi.org/10.3389/fmicb.2019.02650.en_US
dc.description.abstractDespite widespread use in human biology, genome-wide association studies (GWAS) of bacteria are few and have, to date, focused primarily on pathogens. Myxobacteria are predatory microbes with large patchwork genomes, with individual strains secreting unique cocktails of predatory proteins and metabolites. We investigated whether a GWAS strategy could be applied to myxobacteria to identify genes associated with predation. Deduced proteomes from 29 myxobacterial genomes (including eight Myxococcus genomes sequenced for this study), were clustered into orthologous groups, and the presence/absence of orthologues assessed in superior and inferior predators of ten prey organisms. 139 ‘predation genes’ were identified as being associated significantly with predation, including some whose annotation suggested a testable predatory mechanism. Formaldehyde dismutase (fdm) was associated with superior predation of Pseudomonas aeruginosa, and predatory activity of a strain lacking fdm could be increased by the exogenous addition of a formaldehyde detoxifying enzyme, suggesting that production of formaldehyde by P. aeruginosa acts as an anti-predation behaviour. This study establishes the utility of bacterial GWAS to investigate microbial processes beyond pathogenesis, giving plausible and verifiable associations between gene presence/absence and predatory phenotype. We propose that the slow growth rate of myxobacteria, coupled with their predatory mechanism of constitutive secretion, has rendered them relatively resistant to genome streamlining. The resultant genome expansion made possible their observed accumulation of prey-specific predatory genes, without requiring them to be selected for by frequent or recent predation on diverse prey, potentially explaining both the large pan-genome and broad prey range of myxobacteria.en_US
dc.description.sponsorshipGenome sequencing was provided by MicrobesNG (http://www.microbesng.uk) which was supported by the Biotechnology and Biological Sciences Research Council (BBSRC Grant No. BB/L024209/1). IBERS receives strategic funding from the BBSRC.en_US
dc.language.isoenen_US
dc.publisherFrontiers Mediaen_US
dc.relation.ispartofseriesFrontiers in Microbiology;
dc.titleGenome-Wide Identification of Myxobacterial Predation Genes and Demonstration of Formaldehyde Secretion as a Potentially Predation-Resistant Trait of Pseudomonas aeruginosaen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.3389/fmicb.2019.02650
dcterms.dateAccepted2019-10-30
rioxxterms.funderCardiff Metropolitan Universityen_US
rioxxterms.identifier.projectCardiff Metropolian (Internal)en_US
rioxxterms.versionVoRen_US
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/en_US
rioxxterms.licenseref.startdate2020-01-15
rioxxterms.funder.project37baf166-7129-4cd4-b6a1-507454d1372een_US


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