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dc.contributor.authorZwarycz, Allison
dc.contributor.authorLivingstone, Paul
dc.contributor.authorWhitworth, David
dc.date.accessioned2020-06-11T09:55:58Z
dc.date.available2020-06-11T09:55:58Z
dc.date.issued2020-04-29
dc.identifier.citationZwarycz, A.S., Livingstone, P.G. and Whitworth, D.E. (2020) 'Within-species variation in OMV cargo proteins: the Myxococcus xanthus OMV pan-proteome', Molecular Omics.en_US
dc.identifier.issn2515-4184
dc.identifier.urihttp://hdl.handle.net/10369/11067
dc.descriptionArticle published in Molecular Omics on 29 April 2020, available open access at: https://doi.org/10.1039/d0mo00027b.en_US
dc.description.abstractExtracellular membrane vesicles are produced by all domains of life (bacteria, archaea and eukaryotes). Bacterial extracellular vesicles (outer membrane vesicles or OMVs) are produced by outer membrane blebbing, and contain proteins, nucleic acids, virulence factors, lipids and metabolites. OMV functions depend on their internal composition, therefore understanding the proteome of OMVs, and how it varies between organisms, is imperative. Here, we report a comparative proteomic profiling of OMVs from strains of Myxococcus xanthus, a predatory species of Gram-negative myxobacteria whose secretions include secondary metabolites and hydrolytic enzymes, thought to be involved in prey lysis. Ten strains were chosen for study, of which seven had genome sequences available. The remaining three strains were genome sequenced allowing definition of the core and accessory genes and genome-derived proteins found within the pan-genome and pan-proteome respectively. OMVs were isolated from each strain and proteins identified using mass spectrometry. The M. xanthus OMV pan-proteome was found to contain tens of ‘core’ and hundreds of ‘accessory’ proteins. Properties of the OMV pan-proteome were compared with those of the pan-proteome deduced from the M. xanthus pan-genome. On average, 80% of ‘core’ OMV proteins are encoded by genes of the core genome, yet the OMV proteomes of individual strains contain subsets of core genome-derived proteins which only partially overlap. In addition, the distribution of characteristics of vesicle proteins does not correlate with the genome-derived proteome characteristic distribution. We hypothesize that M. xanthus cells package a personalized subset of proteins whose availability is only partially dictated by the presence/absence of encoding genes within the genome.en_US
dc.description.sponsorshipThis work was supported by the Aberystwyth University Research Fund.en_US
dc.language.isoenen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.ispartofseriesMolecular Omics;
dc.titleWithin-species variation in OMV cargo proteins: the Myxococcus xanthus OMV pan-proteomeen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1039/d0mo00027b
dcterms.dateAccepted2020-04-27
rioxxterms.funderCardiff Metropolitan Universityen_US
rioxxterms.identifier.projectCardiff Metropolian (Internal)en_US
rioxxterms.versionVoRen_US
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/3.0/en_US
rioxxterms.licenseref.startdate2020-06-11
rioxxterms.funder.project37baf166-7129-4cd4-b6a1-507454d1372een_US


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