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dc.contributor.authorHeath, Nicola
dc.contributor.authorRowlands, Richard S.
dc.contributor.authorWebster, G.
dc.contributor.authorMahenthiralingam, E.
dc.contributor.authorBeeton, Michael L.
dc.date.accessioned2020-10-02T10:49:03Z
dc.date.available2020-10-02T10:49:03Z
dc.date.issued2020-09-23
dc.identifier.citationHeath, N.L., Rowlands, R.S., Webster, G., Mahenthiralingam, E. and Beeton, M.L. (2020) 'Antimicrobial activity of enacyloxin IIa and gladiolin against the urogenital pathogens Neisseria gonorrhoeae and Ureaplasma spp', Journal of Applied Microbiology. https://doi.org/10.1111/jam.14858en_US
dc.identifier.issn1364-5072
dc.identifier.issn1365-2672 (online)
dc.identifier.urihttp://hdl.handle.net/10369/11160
dc.descriptionArticle published in Journal of Applied Microbiology available open access at https://doi.org/10.1111/jam.14858en_US
dc.description.abstractAims To determine the antimicrobial activity of enacyloxin IIa and gladiolin against N. gonorrhoeae and Ureaplasma spp. Methods and Results The Burkholderia polyketide antibiotics enacyloxin IIa and gladiolin were tested against 14 N. gonorrhoeae and 10 Ureaplasma spp. isolates including multi‐drug resistant N. gonorrhoeae isolates WHO V, WHO X and WHO Z as well as macrolide, tetracycline and ciprofloxacin resistant ureaplasmas. Susceptibility testing of N. gonorrhoeae were carried out by agar dilution, whereas broth micro‐dilution and growth kinetic assays was used for Ureaplasma spp. The MIC range for enacyloxin IIa and gladiolin against N. gonorrhoeae was 0.015 – 0.06 mg/L and 1 ‐ 2 mg/L, respectively. The presence of resistance to front line antibiotics had no effect on MIC values. The MIC range for enacyloxin IIa against Ureaplasma spp. was 4 – 32 mg/L with a clear dose‐dependent effect when observed using a growth kinetic assay. Gladiolin had no antimicrobial activity on Ureaplasma spp. at 32 mg/L and limited impact on growth kinetics. Conclusions Enacyloxin IIa and gladiolin antibiotics have antimicrobial activity against a range of antibiotic susceptible and resistant N. gonorrhoeae and Ureaplasma isolates. Significance and Impact of Study This study highlights the potential for a new class of antimicrobial against pathogens in which limited antibiotics are available. Development of these compounds warrants further investigation in the face of emerging extensively‐drug resistant strains.en_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofseriesJournal of Applied Microbiology;
dc.subjectNovel antimicrobialsen_US
dc.subjectNeisseria gonorrhoeaeen_US
dc.subjectUreaplasma parvumen_US
dc.subjectUreaplasma urealyticumen_US
dc.subjectEnacyloxin llaen_US
dc.subjectgladiolinen_US
dc.titleAntimicrobial activity of enacyloxin IIa and gladiolin against the urogenital pathogens Neisseria gonorrhoeae and Ureaplasma sppen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1111/jam.14858
dcterms.dateAccepted2020-09-14
rioxxterms.funderCardiff Metropolitan Universityen_US
rioxxterms.identifier.projectCardiff Metropolian (Internal)en_US
rioxxterms.versionVoRen_US
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en_US
rioxxterms.licenseref.startdate2021-09-23
rioxxterms.funder.project37baf166-7129-4cd4-b6a1-507454d1372een_US


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