Protective effects of a unique combination ofnutritionally active ingredients on risk factors andgene expression associated with atherosclerosis inC57BL/6J mice fed a high fat diet
Hughes, Timothy R.
Menendez-Gonzalez, Juan B.
Rodrigues, Neil P.
Michael, Daryn R.
Ramji, Dipak P.
Royal Society of Chemistry
MetadataShow full item record
Atherosclerosis, an inflammatory disorder of the vasculature and the underlying cause of cardiovascular disease, is responsible for one in three global deaths. Consumption of active food ingredients such as omega-3 polyunsaturated fatty acids, flavanols and phytosterols has many beneficial effects on cardiovascular disease. However, their combined actions on the risk factors for atherosclerosis remains poorly understood. We have previously shown that a formulation containing each of these active components at physiologically relevant doses modulated several monocyte/macrophage processes associated with atherosclerosis in vitro, including inhibition of cytokine-induced pro-inflammatory gene expression, chemokine-driven monocyte migration, expression of M1 phenotype markers, and promotion of cholesterol efflux. The objectives of the present study were to investigate whether the protective actions of the formulation extended in vivo and to delineate the potential underlying mechanisms. The formulation produced several favourable changes, including higher plasma levels of HDL and reduced levels of macrophages and myeloid-derived suppressor cells in the bone marrow. The mRNA expression of liver-X-receptor-α, peroxisome proliferator-activated receptor-γ and superoxide dismutase-1 was induced in the liver and that of interferon-γ and the chemokine (C–X–C motif) ligand 1 decreased, thereby suggesting the potential mechanisms for many beneficial effects. Other changes were also observed such as increased plasma levels of triglycerides and lipid peroxidation that may reflect potential activation of brown fat. This study provides new insights into the protective actions and the potential underlying mechanisms of the formulation in vivo, particularly in relation to risk factors together with changes in systemic inflammation and hepatic lipid alterations associated with atherosclerosis and metabolic syndrome, and supports further assessments in human trials.
Food & Function;
Moss, J.W., Williams, J.O., Al-Ahmadi, W., O'Morain, V., Chan, Y.H., Hughes, T.R., Menendez-Gonzalez, J.B., Almotiri, A., Plummer, S.F., Rodrigues, N.P. and Michael, D.R. (2021) 'Protective effects of a unique combination of nutritionally active ingredients on risk factors and gene expression associated with atherosclerosis in C57BL/6J mice fed a high fat diet', Food & Function. https://doi.org/10.1039/D0FO02867C
Article published in Food & Function available open access at https://doi.org/10.1039/D0FO02867C
Showing items related by title, author, subject and abstract.
Alhadidi, Younis (Cardiff Metropolitan University, 2011)Objective: Conjugated linoleic acid (CLA) is a group of naturally occurring fatty acids found in food of dairy products in ruminated animals. CLA found to have beneficial effects in animal models as it has anti-atherosclerotic ...
Al-Shukaili, Nasser (Cardiff Metropolitan University, 2011)The role of dietary factors in the prevention of atherosclerosis has been the focus of important attention. The classic example is conjugated linoleic acids CLA are a mixture of isomers of linoleic acid found mainly in the ...
The effect of Conjugated Linoleic Acid and its 9:11 and 10:12 isomers on the inflamatory profiles on monocytic / macrophage and endothelial cell systems Al-Muharrami, Khalid (University of WalesCardiff School of Health Sciences, 2010)This study investigated the role of the dietary lipid Conjugated Linoleic Acid (CLA) as a 50:50 mixture of 9:11 CLA and 10:12 CLA and these two isoforms used individually, in regulating the carboxymethyllysine (CML) induced ...