Molecular exploration for Mycoplasma amphoriforme, Mycoplasma fermentans and Ureaplasma spp. from patient samples previously investigated for Mycoplasma pneumoniae infection
Rowlands, Richard S.
Beeton, Michael L.
MetadataShow full item record
Objectives: To determine the presence and genotypic macrolide susceptibility of Mycoplasma amphoriforme, and presence of Ureaplasma spp., and Mycoplasma fermentans among clinical samples from England previously investigated for Mycoplasma pneumoniae. Methods: Quantitative and conventional PCR were used to retrospectively screen a collection of 160 clinical samples, previously submitted to Public Health England (PHE) for detection of M. pneumoniae, between October 2016 and December 2017. Samples which were positive for M. amphoriforme DNA were further investigated for mutations associated with genotypic macrolide resistance by sequencing of domain V of the 23s rRNA. Results: Mycoplasma amphoriforme were detected in 10/160 (6.3%) samples, Ureaplasma parvum were detected in 4/160 samples (2.5%) with 0/160 M. fermentans detections. Of the nine individuals (two samples were from the same patient) in which M. amphoriforme were detected, eight were male (10 – 60 years age range) and one was female (30 – 40 years age range). One individual, with cystic fibrosis was positive for both M. amphoriforme and U. parvum. All M. amphoriforme DNA were genotypically susceptible for macrolides. Conclusions: Mycoplasma amphoriforme were found in clinical samples including the lower respiratory tract samples of patients with pneumonia. In the absence of other respiratory pathogens, these data suggest a potential role of this organism in human disease, with no evidence of acquired macrolide resistance. Ureaplasma parvum was detected in cerebrospinal fluid and respiratory tract samples. These data suggest the need to consider these atypical respiratory pathogens in future diagnostic investigations.
Clinical Microbiology and Infection;
Rehman, S.U., Day, J., Afshar, B., Rowlands, R.S., Billam, H., Joseph, A., Guiver, M., Maddocks, S.E., Chalker, V.J. and Beeton, M.L., (2021) 'Molecular exploration for Mycoplasma amphoriforme, Mycoplasma fermentans and Ureaplasma spp. from patient samples previously investigated for Mycoplasma pneumoniae infection', Clinical Microbiology and Infection. https://doi.org/10.1016/j.cmi.2021.06.021
Article published in Clinical Microbiology and Infection available at https://doi.org/10.1016/j.cmi.2021.06.021
Cardiff Metropolitan University (Grant ID: Cardiff Metropolian (Internal))
Showing items related by title, author, subject and abstract.
Beeton, Michael L.; Zhang, Xu-Sheng; Uldum, Søren A.; Bébéar, Cécile; Dumke, Roger; Gullsby, Karolina; Ieven, Margareta; Loens, Katherine; Nir-Paz, Ran; Pereyre, Sabine; Spiller, O. Brad; Chalker, Victoria J.; ESCMID Study Group for Mycoplasma and Chlamydia Infections (ESGMAC) Mycoplasma pneumoniae sub-group (European Centre for Disease Prevention and Control, 2020-01-16)Background: Mycoplasma pneumoniae is a leading cause of community-acquired pneumonia, with large epidemics previously described to occur every 4 to 7 years. Aim: To better understand the diagnostic methods used to ...
An evaluation of some microbiological and ATP bioluminescence methods for the recovery and detection of bacterial contamination from food contact and environmental surfaces Davidson, Craig A. (Cardiff Metropolitan University, 2001)Plant hygiene and food contact surface cleanliness are key prerequisites to the management of food quality and safety, and may form a critical control point within Hazard Analysis Critical Control Point food safety management ...
Beeton, Michael L.; Spiller, Owen (Oxford University Press, 2016-10-20)There is growing global concern regarding the rise of antibiotic-resistant organisms. Many of these reports have focused on various Gram-positive and Gram-negative pathogens, with little attention to the genus Ureaplasma. ...