• English
    • Welsh
  • English 
    • English
    • Welsh
  • Login
Search DSpace:
  • Home
  • Research at Cardiff Met
  • Library Services
  • Contact Us
View item 
  • DSpace home
  • Cardiff School of Sport and Health Sciences
  • Cardiovascular Health and Ageing
  • View item
  • DSpace home
  • Cardiff School of Sport and Health Sciences
  • Cardiovascular Health and Ageing
  • View item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Chronic oxidative stress compromises telomere integrity and accelerates the onset of senescence in human endothelial cells

Thumbnail
Author
Kurz, D. J.
Decary, S.
Hong, Y.
Trivier, E.
Akhmedov, A.
Erusalimsky, Jorge
Date
2004
Publisher
The Company of Biologists
ISSN
0021-9533
Metadata
Show full item record
Abstract
Replicative senescence and oxidative stress have been implicated in ageing, endothelial dysfunction and atherosclerosis. Replicative senescence is determined primarily by telomere integrity. In endothelial cells the glutathione redox-cycle plays a predominant role in the detoxification of peroxides. The aim of this study was to elucidate the role of the glutathione-dependent antioxidant system on the replicative capacity and telomere dynamics of cultured endothelial cells. Human umbilical vein endothelial cells were serially passaged while exposed to regular treatment with 0.1 μM tert-butyl hydroperoxide, a substrate of glutathione peroxidase, or 10 μM L-buthionine-[S,R]-sulphoximine, an inhibitor of glutathione synthesis. Both treatments induced intracellular oxidative stress but had no cytotoxic or cytostatic effects. Nonetheless, treated cultures entered senescence prematurely (30 versus 46 population doublings), as determined by senescence-associated β-galactosidase staining and a sharp decrease in cell density at confluence. In cultures subjected to oxidative stress terminal restriction fragment (TRF) analysis demonstrated faster telomere shortening (110 versus 55 bp/population doubling) and the appearance of distinct, long TRFs after more than 15-20 population doublings. Fluorescence in situ hybridisation analysis of metaphase spreads confirmed the presence of increased telomere length heterogeneity, and ruled out telomeric end-to-end fusions as the source of the long TRFs. The latter was also confirmed by Bal31 digestion of genomic DNA. Similarly, upregulation of telomerase could not account for the appearance of long TRFs, as oxidative stress induced a rapid and sustained decrease in this activity. These findings demonstrate a key role for glutathione-dependent redox homeostasis in the preservation of telomere function in endothelial cells and suggest that loss of telomere integrity is a major trigger for the onset of premature senescence under mild chronic oxidative stress.
Journal/conference proceeding
Journal of Cell Science
Citation
Journal of Cell Science, 117 (11), pp.2417-2426
URI
http://hdl.handle.net/10369/325
DOI
http://dx.doi.org/10.1242/jcs.01097
Collections
  • Cardiovascular Health and Ageing [139]

Related items

Showing items related by title, author, subject and abstract.

  • Thumbnail

    SIRT6 protects human endothelial cells from DNA damage, telomere dysfunction, and senescence 

    Cardus, Anna; Uryga, Anna; Walters, Gareth; Erusalimsky, Jorge (Oxford University Press, 2013)
    AIMS: Although endothelial cell senescence is known to play an important role in the development of cardiovascular pathologies, mechanisms that attenuate this process have not been extensively investigated. The aim of this ...
  • Thumbnail

    The angiotensin-(1-7)/Mas receptor a xis protects from endothelial cell senescence via klotho and Nrf2 activation 

    Romero, Alejandra; Hipólito-Luengo, Álvaro San; Villalobos, Laura; Vallejo, Susana; Valencia, Inés; Michalska, Patrycja; Pajuelo-Lozano, Natalia; Sánchez-Pérez, Isabel; León, Rafael; Bartha, José Luis; Sanz, María J.; Erusalimsky, Jorge; Sánchez-Ferrer, Carlos F.; Romacho, Tania; Peiró, Concepción (Wiley, 2019)
    Endothelial cell senescence is a hallmark of va scular aging that pre disposes to vascular disease. We aimed to explore the capacity of the renin-angiotensin system (RAS) heptapeptide angiotensin ...
  • Thumbnail

    Cellular senescence after single and repeated balloon catheter denudations of rabbit carotid arteries 

    Fenton, M.; Barker, S.; Kurz, D. J.; Erusalimsky, Jorge (American Heart Association, 2001)
    The hypothesis that increased cellular proliferation in the vasculature may lead to replicative senescence has been tested in a model of neointima formation. We have used a biomarker of replicative senescence, senescence ...

Browse

DSpace at Cardiff MetCommunities & CollectionsBy issue dateAuthorsTitlesSubjectsThis collectionBy issue dateAuthorsTitlesSubjects

My Account

Login

Statistics

Most Popular ItemsStatistics by CountryMost Popular Authors

DSpace software copyright © 2002-2015  DuraSpace
Contact us | Send feedback | Administrator