• English
    • Welsh
  • English 
    • English
    • Welsh
  • Login
Search DSpace:
  • Home
  • Research at Cardiff Met
  • Library Services
  • Contact Us
View item 
  • DSpace home
  • Theses
  • PhD theses \ Traethodau PhD
  • View item
  • DSpace home
  • Theses
  • PhD theses \ Traethodau PhD
  • View item
JavaScript is disabled for your browser. Some features of this site may not work without it.

In-Vitro Investigation on the Protective Effect of Glycomacropeptide against Acid Erosion of Tooth Enamel

Thumbnail
View/open
Anita Setarehnejad.pdf (1.481Mb)
Author
Setarehnejad, Anita
Date
2011
Type
Thesis
Publisher
University of Wales
Metadata
Show full item record
Abstract
Tooth enamel is built of crystalline calcium phosphate fixed into a protein matrix. When acid comes into contact with tooth surface it encourages demineralisation and erosion of tooth enamel. Eroded tooth with small cavities becomes vulnerable to the attack of microflora present in dental plaque on tooth surfaces and increases the risk of tooth decay. It has been reported that dental erosion and caries among children and adults have an increasing trend. It is thought that changes in drinking habit and more interest in consumption of soft drinks could be a possible reason for this dental health problem. To reduce the risk of dental erosion several studies were carried out and some research indicated that milk proteins and peptides have anti-erosive and anti-caries effect. In this category casein phosphopeptides (CPP) a tryptic hydrolysate of casein was widely studied to reduce dental problems. In addition, glycomacropeptide (GMP), a hydrophilic part of κ-casein released in whey, was of interest. In this study, attempts were made to reduce the mineral dissolution of enamel using GMP and GMP fractions during different acid contact times. To simulate the mineral dissolution of enamel hydorxyapatite (HA, calcium phosphate) was used. At different experiments HA was treated with GMP prior to exposure to 0.1M citrate buffer at different pH levels of 2.5-4.5. The level of dissolved calcium and phosphate into citrate buffer was measured by atomic absorption spectroscopy and Allen‘s colorimetric method, respectively. The erosive effect of soft drinks was investigated using orange juice and coca-cola containing GMP and its protection level was determined by measuring the amount of dissolved minerals in soft drinks compared to a control. To examine the protective effect of the GMP from acid attack on enamel surface, human enamel was studied by scanning electron microscopy. This study showed that GMP could reduce dental erosion and act better than CPP at low pH (3.0). This protective effect of GMP against mineral dissolution of enamel could be due to its attachment to the surface, its amino acid sequence and glycosylation level and/or to the overall net charge of the peptide.
URI
http://hdl.handle.net/10369/3270
Collections
  • PhD theses \ Traethodau PhD [469]

Related items

Showing items related by title, author, subject and abstract.

  • Thumbnail

    Investigation of the erosive potential of sour novelty sweets 

    Aljawad, A.; Morgan, M.Z.; Fairchild, Ruth M.; Rees, J.S. (Nature, 2017-04-21)
    Provides a background about the link between acidic beverages and dental erosion. Discusses the potential risk of developing dental erosion upon the frequent consumption of novelty sweets. Provides information which could ...
  • Thumbnail

    The protective effect of fluoride against the erosion of dental hard tissue 

    Hughes, J.A. (Cardiff Metropolitan University, 1996)
    The erosive effect of acidic soft drinks on tooth tissue has been frequently reported in the literature (Eccles and Jenkins 1974). The phenomena is believed to be increasing due to the “health diet" and longevity of the ...
  • Thumbnail

    An anterior ankle-foot orthosis improves walking economy in Charcot–Marie–Tooth type 1A patients 

    Menotti, F.; Laudani, Luca; Damiani, A.; Mignogna, T.; Macaluso, A. (SAGE, 2014-10)
    Background: Ankle-foot orthoses are commonly prescribed in Charcot–Marie–Tooth type 1A disease to improve quality of walking and reduce the risk of falling due to the foot drop. Objectives: This study aimed at assessing ...

Browse

DSpace at Cardiff MetCommunities & CollectionsBy issue dateAuthorsTitlesSubjectsThis collectionBy issue dateAuthorsTitlesSubjects

My Account

Login

Statistics

Most Popular ItemsStatistics by CountryMost Popular Authors

DSpace software copyright © 2002-2015  DuraSpace
Contact us | Send feedback | Administrator