The effect of Theaflavins and EGCG on the Cervical Cancer Cell line HT-3
University of Wales Institute Cardiff
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Cervical cancer is the second most common cancer in women. The presence or absence of HPV DNA is one difference detected between cervical cancer cell lines, and suggested, is that this difference in genetics of cell line could result in different outcomes when treated with chemopreventative drugs. Research has suggested the use of the commonly consumed beverage tea, as a chemopreventative drug, as previous investigations have suggested that the polyphenols contained within it may prevent cancer. Although the black tea components, theaflavins have been tested as inducers of apoptosis in HeLa cells, to date, theaflavins have not been assessed as effectors of HT-3 cervical cancer cell viability or proliferation. This project examines the effect of theaflavins on HT-3 cells and compares the effects produced with that produced by EGCG. Increasing concentrations of both theaflavins and EGCG was found to decrease the number of viable HT-3 cells; a theaflavin concentration at 80ug/ml and an EGCG concentration at 60ug/ml showed no viable HT-3 cells, thus EGCG proved a more successful inhibitor of HT-3 cell proliferation than theaflavins. Previous investigation into theaflavins found them to inhibit at HeLa cells at 110μg/ml, and for the first time, it was discovered that theaflavins were able to inhibit the proliferation of HT-3 cells at 80ug/ml; Theaflavins were found to be more apoptotic to squamous carcinoma cells than to cervical adenocarcinoma cells. Both theaflavins and EGCG were identified as inducing apoptosis in HT-3 cells in this investigation, there is therefore reason to further investigate the mechanisms of both theaflavins and EGCG as anti-proliferative tea components to other cell lines.
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