The effect of honey extract on LPS induced NF-κB activation in the HaCat cell line
University of Wales Institute Cardiff
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Since ancient time, honey has been used for both nutritional and as a medicine. A lot of studies support the beneficial effects of honey in many chronic inflammatory diseases. Honey extract contain different amount of phenolic compounds and other constants which have different anti-inflammatory activates. The purpose of the present study was to investigate the effect of honey extract on TNF induced NF-κB activation in HaCat cells. Also, it hypothesized that honey can inhibited of NF-κB activation by PPARs activation, as the honey contain PPARs ligands and thus enhance PPAR transcriptional activity. In this study various concentration of honey extract were used (1% and 5%) and tested in vitro in HaCaT cells and demonstrate their effect on TNF induced NF-κB activation, for this purpose the cytokine IL-8 and MCP-1 concentration were measured in pre-treated and immediate HaCaT cells with 1% and 5% of honey extract, by using ELISA assay. The result showed that, pre-treated and immediate treatment of HaCaT cells with 1% of manuka honey extract lead to down regulation of IL-8 , while in MCP-1 the inhibition were partially ( where in pre-treated HaCat cells at 50 and 100 ng/ml of TNF, the concentration were increased). On the other hand, the 5% of honey extract enhanced the production of IL-8 in pretreated HaCaT cells, while in immediate treatment the 5% of honey extract lead to reduced IL-8 (except at 50ng/ml of TNF) and MCP-1. Taken the result to gather, from the result obtained in this study, this finding suggested that manuka honey extract with low concentration has anti-inflammatory effect in TNF induced NF-κB and this could be explained by activation of PPARs which leads to inhibition of NF-ΚB as a result this leading to inhibit the production of cytokines IL-8 and MCP-1.
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