The effect of manuka honey and the iron chelator 2, 2-dipyridyl on biofilm biomass of S. aureus, P. aeruginosa, and S. pyogenes
University of Wales Institute Cardiff
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Bacterial biofilms are the main causes of infection in many cases of sever non healing wound infections. They form this community as a protective way to survive when there are not enough nutrients, and pH and temperature of the environment is appropriate to grow and multiply. In this study the effect of manuka honey and iron chelator on the biofilms of P.aeroginosa, S.aureus, and, S.pyogenes was investigated. All these bacteria were able to grow and multiply at 37ºC when growth curve was done within five hours of incubation. When the iron chelators DIP were added there was no effect on P. aeruginosa or S. aureus but it inhibited the growth of S. pyogenes. DIP had very little effect on biofilms. When manuka honey used at a concentration of 20% it did not inhibit the growth of all these bacteria when they are in their free living plankotonic but when we use it with the biofilm of these bacteria honey shows an inhibitory effect on all these bacteria at same concentration. The MIC for S.aureus and S.pyogenes was 15%, and for P.aeuroginosa it was 20%, while the MBC for both S.aureus and S.pyogenes was also 15% which is same as the MIC. And for P.aeuroginosa was also 15% which is 5% less than the MIC this indicate that honey has an inhibitory effect when it used at right concentration to treat the biofilm of these bacteria this was also proved by the TVC. When DIP and manuka honey were used together for growth curved the DIP stopped the effect of manuka honey on growth for P. aeruginosa and S. aureus but not for S. pyogenes. In biofilms the DIP increased biofilm biomass even when bacteria were treated with honey for S. aureus. But it had no effect on S. pyogenes that were treated with honey and no effect of P. aeruginosa that were treated with honey.
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