Al Bulushi, Mohamed
University of Wales Institute Cardiff
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Introduction: bacterial Biofilms often consist of multi-species of bacteria which are called as polymicrobial for example in chronic wounds. There are dynamical changes of biofilm colonies in polymicrobial type of growth involves competition to grow as co-culture and co-existence nature growth. Method: Streptococcus pyogenes (MGAS6180), Staphylococcus aureus (NCTC10017) and Pseudomonas aeruginosa (NCIMB8626) biofilms were grown individually and optimised to best size of inoculum and length of incubation in micro-titre plates by microscopy, which were the preliminary experiments. Using optimised feature of growth, 12 sets of polymicrobial growth carried out with various ratios among the three bacteria that were determined by TVC and gram stain to study the growth rate. Results: S. aureus and P. aeruginosa formed biofilm microcolonies in 24 h culture where S. pyogenes took 48 h, however 60ul of bacterial culture was best dose size. P. aeruginosa found to have high growth rate, which revealed a significant number of viable cells followed by S. pyogenes and then Staphylococcus aureus. Conclusion: The mixed species biofilm dynamic and development shows a co-existence pattern of growth, however ratio of microorganism in polymicrobial community reflect the number of bacteria in early biofilm development state. Although P. aeruginosa have best growth rate but it couldn’t exclude other strains of bacteria from polymicrobial biofilm community. All of these findings can be used in further investigations, to compare mixed species biofilm state with more strains of bacteria.
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