The role of human tissue proteins in mediating aggregation and biofilm development in Streptococcus spp.
Al Shamsi, Iman Khamis Mohammed
University of Wales Institute Cardiff
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Streptococcus pyogenes (group A Streptococcus; GAS) is always of clinical significance in wounds where it can initiate infection. Streptococcus gordonii (part of mitis group of streptococci) is a normal flora of human oral cavity. Both of these organisms can cause infections to the human and persist as a biofilm. Manuka honey is a well-known antimicrobial and has broad spectrum antimicrobial activity. In this study, the effect of manuka honey on Streptococcus pyogenes (group A streptococcus; GAS) and Streptococcus gordonii were investigated in vitro with planktonic cells using growth curve, minimum inhibitory concentration (MIC) and, minimum bactericidal concentration (MBC). The effect of fibrinogen and collagen on bacterial aggregation was also investigated, as was the potential for manuka honey to disrupt this process. The manuka honey inhibited the planktonic growth of both organisms at certain concentrations. For examples; S. pyogenes at 25% of manuka honey there was no growth while S. gordonii at 15% was no growth (MBC). Fibrinogen and collagen mediated aggregation of S. pyogenes and manuka honey stopped the aggregation by fibrinogen. These finding indicate that manuka honey has an effect on S. pyogenes and S. gordonii and can be used as antimicrobial or anti-adhesive treatment.
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