An Initial Evaluation Of The Potential For Inhibition Of Siderophores By Medical Honey
University of Wales Institute Cardiff
MetadataShow full item record
Introduction The antibacterial effects and mode of action of medical honeys such as the increasingly well known manuka honey are currently under investigation. In order for these to be considered for use as a mainstream antibacterial agent further understanding is required. Major factors such as methylglyoxal, hydrogen peroxide and bee defensin-1 have been the subject of research but other factors remain to be isolated and characterised. This investigation was performed to determine if any of the factors in a branded medical honey had an effect on the siderophore production of three common bacterial pathogens. In recent years siderophore inhibition has been investigated as a viable method of inhibiting bacterial growth and this seems to be a potentially profitable avenue of research. Methods Three bacterial strains, Staphylococcus aureus (NCTC10017), Streptococcus pyogenes (MGAS6180) and Pseudomonas aeruginosa (NCIMB8626) were selected for this investigation due to their presence as common human pathogens as well as their status as having characterised siderophores and methods of iron acquisition. Each of these strains were cultured in subinhibitory concentrations of medical honey (Comvita Medihoney) and their subsequent siderophore production assayed using the Chrome Azurol S universal siderophore assay as described by Schwyn and Neilands. These results were then compared to those of cultures not exposed to the honey. Results Strep. pyogenes showed no noticeable change in siderophore production as expected due to it not producing any known siderophores. Both S. aureus and Ps. aeruginosa demonstrated lower production of siderophores when grown under exposure of honey compared to the control cultures. However convincing conclusions could not be drawn about which bacteria was effected to a greater extent or how the inhibiting action of honey compares to specifically targeted anti-siderophore agents. Conclusion The ability of medical honey to inhibit siderophore production seems promising but further investigation into how this effect is induced and whether the extent of this inhibition is a contributing factor to the antibacterial effect of medical honey.
Showing items related by title, author, subject and abstract.
Honey as an effective antimicrobial treatment for chronic wounds: is there a place for it in modern medicine? Cooper, Rose (Dove Press, 2014-08-06)Honey has a long history in the treatment of wounds, where claims of its therapeutic properties include the inhibition of a wide range of infectious agents as well as an ability to promote rapid wound healing. However, ...
Al-Khanbashi, Laila (University of Wales Institute Cardiff, 2012)Since ancient time, honey has been used for both nutritional and as a medicine. A lot of studies support the beneficial effects of honey in many chronic inflammatory diseases. Honey extract contain different amount of ...
Henriques, Ana (University of Wales, 2006)Honey is an old remedy recently rediscovered as a possible alternative to modern antibiotics in wound management but its mode of action is not fully understood. The antibacterial activity of honey can be divided into ...