• English
    • Welsh
  • English 
    • English
    • Welsh
  • Login
Search DSpace:
  • Home
  • Research at Cardiff Met
  • Library Services
  • Contact Us
View item 
  • DSpace home
  • Cardiff School of Sport and Health Sciences
  • Cardiovascular Health and Ageing
  • View item
  • DSpace home
  • Cardiff School of Sport and Health Sciences
  • Cardiovascular Health and Ageing
  • View item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Dipalmitoylphosphatidylcholine modulates inflammatory functions of monocytic cells independently of mitogen activated protein kinases

Thumbnail
Author
Tonks, Alex
Morris, Keith
Price, A.J.
Thomas, Andrew
Jones, Ken
Jackson, S.K.
Date
2001
Publisher
British Society for Immunology
ISSN
1365-2249
Metadata
Show full item record
Abstract
Phosphatidylcholine (PC) is the major phospholipid of pulmonary surfactant and it is hypothesized that PC and its subspecies modulate the functions of alveolar macrophages. The most abundant of these subspecies is dipalmitoylphosphatidylcholine (DPPC). This study was undertaken to determine the effect of PC on monocyte function using a human monocytic cell line, MonoMac-6 (MM6). This study showed that preincubation of MM6 cells with DPPC at 125 μg/ml for 2 h inhibited the oxidative response to either zymosan or phorbol-12-myristate-13-acetate (PMA) by 30% (P < 0·001). This inhibition with DPPC was independent of LPS priming. When DPPC was replaced with 1-palmitoyl-2-arachidonoyl phosphatidylcholine (PAPC) there was no inhibition and in contrast a significant increase in oxidant production was observed. We also demonstrated that total PC (tPC; a heterogeneous species of PC from egg) and DPPC but not PAPC significantly inhibited the release of TNF-α from MM6 cells (P < 0·05). DPPC did not inhibit phosphorylation of the mitogen activated protein kinases (MAPKs) p44/p42 or p38 in stimulated cells. Measurements of membrane fluidity with spin label EPR spectroscopy indicate that DPPC incorporation significantly alters the membrane fluidity of MM6 cells. These results suggest that DPPC, the major component of pulmonary surfactant, may play a role in modulating leucocyte inflammatory responses in the lung. This may in part be related to membrane effects but does not include alterations in p44/p42 or p38 MAPK signalling.
Journal/conference proceeding
Clinical & Experimental Immunology
Citation
Tonks, A., Morris, R.H.K., Price, A.J., Thomas, A.W., Jones, K.P. and Jackson, S.K. (2001) 'Dipalmitoylphosphatidylcholine modulates inflammatory functions of monocytic cells independently of mitogen activated protein kinases', Clinical & Experimental Immunology, 124(1), pp.86-94.
URI
http://hdl.handle.net/10369/344
DOI
http://dx.doi.org/10.1046/j.1365-2249.2001.01479.x
Description
This article was published in Clinical & Experimental Immunology on 12 January 2002 (online), available at http://dx.doi.org/10.1046/j.1365-2249.2001.01479.x
Collections
  • Cardiovascular Health and Ageing [156]

Related items

Showing items related by title, author, subject and abstract.

  • Thumbnail

    The effects of whitening toothpastes and agents on extrinsic stain: studies in vitro 

    Sharif, Nusret (Cardiff Metropolitan University, 2002)
    Background - There are various toothpaste products, available on the market, labelled “whitening". Most of these appear to contain ingredients to inhibit or remove extrinsic stains rather than change natural tooth colour. ...
  • Thumbnail

    The Effect of Theaflavins and EGCG on the HT-3 Cervical Cancer Cell Line 

    Meilak, Joseph (University of Wales Institute Cardiff, 2012)
    The health benefits of tea and its constituents have been reported, many being related to the flavonoid content of the tea, with green and black teas containing different types. The theaflavins in black tea are of particular ...
  • Thumbnail

    An Initial Evaluation Of The Potential For Inhibition Of Siderophores By Medical Honey 

    John, David (University of Wales Institute Cardiff, 2012)
    Introduction The antibacterial effects and mode of action of medical honeys such as the increasingly well known manuka honey are currently under investigation. In order for these to be considered for use as a mainstream ...

Browse

DSpace at Cardiff MetCommunities & CollectionsBy issue dateAuthorsTitlesSubjectsThis collectionBy issue dateAuthorsTitlesSubjects

My Account

Login

Statistics

Most Popular ItemsStatistics by CountryMost Popular Authors

DSpace software copyright © 2002-2015  DuraSpace
Contact us | Send feedback | Administrator