Investigating the effects of EGCG on cell proliferation and surface expression of CD55 and CD59 in breast cancer model cell lines MCF-7 and MIDA MB-231
Mehrjou, Craig Parviz
Cardiff Metropolitan University
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Epigallocatechin-3-gallate (EGCG), one of the major catechins in green tea, is a potential chemopreventive agent for a variety of cancers. The aim of this study was to examine the effects of EGCG on proliferation and cellular expression of membrane bound complement proteins (mCRPs) CD55 and CD59 in breast cancer model cell lines MCF-7 and MDA MB-231. Both cell lines showed approximately 50% growth inhibition after incubation with 100µg/ml of EGCG, however MCF-7 cells appeared more sensitive at lower concentrations (< 50µg/ml). Flow Cytometry was used to assess mCRP expression in response to EGCG. The cell lines generated inverse data for complement protein expression, for example MCF-7 cells showed a significant increase in CD59 expression (P = < 0.00023 at 168 hours), where as MDA MB-231 cells generated a significant decrease in cellular expression (P = < 0.00011 at 168 hours). The results obtained for CD55 expression also produced significant inverse results. Cytotox and Caspase assays were performed on cell line MCF-7 to determine if the observed proliferation inhibition was attributable to apoptosis or necrosis. The data generated indicated that 48 hour incubation produced the greatest levels of apoptotic viable cells (P = < 0.0002 at above optimum concentration). It is envisaged that treatment of cancer patients with mCRP inhibition targeted specifically, maybe through the use of EGCG, to cancer cells in combination with anticancer complement-fixing antibodies will improve the therapeutic efficacy. For this fact this work has indicated that the design, investigation and possible use of EGCG and other GTP derivatives may prove a positive association in the overall treatment of cancer.
M.Sc. Biomedical Science
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