Investigating the level of expression of the membrane bound complement regulatory proteins CD55 and CD59 in CACO-2 colon cancer cells exposed to green tea extract Epigallocatechin 3-gallate (EGCG).
Lutfi, Ahmad Kalid
Cardiff Metropolitan University
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Colon cancer is the third most common cancer in the United Kingdom and fourth commonest worldwide. A variety of model systems have been employed to investigate this disease, one of which comprises the CACO-Z epithelial colorectal adenocarcinoma cell line, which has been used in the current study. Tea and a variety of its components have shown to have a variety of beneficial effects, however, their effects on the regulators of immune system and specifically complement proteins CD59 and CD55 have yet to be determined. Therefore the current study was undertaken to investigate the level of cell expression of membrane bound complement proteins CD59/CD55 in CACO-2 cells exposed to the tea polyphenol EGCG over a period of 3 days in addition to cell proliferation and death using the techniques of tissue culture and flowcytometry. Results indicated that EGCG has the capacity to inhibit proliferation of CACO-2 cells and results in cell death. Additionally EGCG was found to initially increase the expression of CD59 by 30% up to 48 hours but thereafter results in a decreased expression between 48 and 72 hours by 35%. Interestingly, EGCG has the capacity to slightly decrease (8%) in CD55 expression up to 48 hours but thereafter increase (30%) the expression to 72 hours. This data suggest that EGCG can initially render the cells more resistant to attack by (MAC) of complement but additionally renders the cells susceptible to lyses by complement system through C3 convertase. Further incubation to 72 hours causes an inversion relationship resulting in phenotype of cells which are more susceptible to lyses by (MAC) and increase resistance to lyses through C3 convertase. This suggests that CACO-2 cells are capable of adapting their regulatory capacity to complement to C3 convertase and MAC lyses steps by exposure to EGCG. Further investigation will reveal the mechanisms involved in these processes and whether this approach will facilitate treatment of colon cancer using EGCG as an adjuvant therapeutic intervention strategy.
MSc Biomedical Sciences
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