The effect of CLA and CML induced RAGE surface expression in THP1 monocyte cells with a view to Diabetes and Inflammation
Curry, Antony James
Cardiff Metropolitan University
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The receptor for advanced glycated end products (RAGE) is thought to have a vital role to play in the future treatment of chronic inflammation and diabetes mellitus, as it has been linked to the inflammatory pathways. Advanced glycated end produces (AGE) are found naturally within the human body, but are found in much higher levels in chronically uncontrolled diabetes mellitus. Also most of the chronic complications of diabetes can be linked to chronic inflammation. It is thought that AGE's bind to RAGE and cause an increase in the cell inflammatory pathway which is responsible for the chronic complications of diabetes. Due to RAGE's central role in monocyte activation, if more could be learnt about how it is activated and regulated it would be of great clinical significance, which is what this study aims to achieve. In investigating this cause, THPI monocytes were grown up, and certain samples had cell stimulants added to them (CML, CLA, LPS). This was done at time points of 24, 48, 72 and 144hrs, through 2 experiments. This allowed us to then flurochrome antibody label both the "normal" and stimulant treated cells, to give off a detective signal. When the cells were effectively labelled the cells were read using a Flow Cytometer, and the Signal given off was recorded as an X mean. The X means were then used to create Graphs of the results. From these results statistical analysis was done, but did not support the differences displayed within the results and therefore we cannot conclude any conclusive results from this experiment. However theoretically some of our analysis dos seem to support other evidence that RAGE expression is influenced by chronic exposure to cell stimulants.
BSc (Hons) Biomedical Science
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