The role of conjugated linoleic acid in regulating of CD206 expression in monocytic cells.
Cardiff Metropolitan University
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Objective: Conjugated linoleic acid (CLA) is a group of naturally occurring fatty acids found in food of dairy products in ruminated animals. CLA found to have beneficial effects in animal models as it has anti-atherosclerotic properties and have gained a considerable attention due to the property of anti-inflammatory activity effects in human cells. There is increasing interest in the role of CLA and rosiglitazone (a member of thiazolinedione class of drugs, and acts as anti-diabetic drug) in prevention and treatment of atherosclerosis and T2D. Activation of nuclear receptor PPARy is linked to the beneficial effects of inflammation, atherosclerosis, Type 2 Diabetes (T2D) and lipid homeostasis. These beneficial effects include differentiating circulating monocytes toward M2 macrophages (anti-inflammatory phenotype). Recently, we have shown that treating differentiated THP-I cells with different concentration of CLA and rosiglitazone (20uM, 100uM CLA and 1uM rosiglitazone) increase PPARy expression and alter macrophages differentiation toward M2 macrophages with presence of IL-13. The present study aimed to observe the effects of CLA and rosiglitazone on differentiated THP-1 cells with and without presence of IL-13. Methods: Differentiated THP-1 cells were treated with 20uM, 100uM CLA and 1uM rosiglitazone before and after polarization to M2 macrophages. The cells were incubated for 72 hours under tissue culture condition. Finally, the effects of different concentration of CLA and rosiglitazone were observed by measuring the expression of CD206 in differentiated THP-I cells by flowcytometry and the level of cytokines (IL-8 and TNFα) were measured by ELISA. Results: different concentration of CLA increased the expression of CD206 in differentiated THP1 cells and in M2 polarized macrophages with presence of IL-13. Tumour necrosis factor alpha TNFα was inhibited by different concentration of CLA and probably down regulated by IL-13. The level of IL-8 cytokine was increased after treating THP-I cells with CLA and rosiglitazone. Thus CLA may have a role in the activation of PPARy and therefore regulating the biological function of the cells and inflammation by increasing certain cytokines such as IL-8, IL-4 and IL-10 while inhibiting others such as TNFα. Conclusion: The effects of CLA and rosiglitazone was reported to activate PPARy and increase the expression of CD206 in differentiated THP1 cells, moreover CLA suppressed the production of TNFα and increased the production of IL-8. On the other hand, IL-3 increased M2 polarization. I propose that CLA and rosiglitazone induced PPARy mediated M2 polarization and produced anti-inflammatory cytokines thus may constitute noval benefits as it has anti-inflammatory properties.
B.Sc. (Hons) Biomedical Science
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