The expression of TNF-alpha in M2 polarised THP-1 monocytes following pre and post stimulationwith conjugated Linoleic acid (CLA)
Oladokun Olawale, Zainab
Cardiff Metropolitan University
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Inflammation caused by inflammatory mediators such as cytokines in the vasculature, is a crucial pathogenic link between cardiovascular diseases and metabolic syndrome. Inflammation can be reducing by approaches that have included diet, exercise, cardiovascular drugs, and insulin sensitizers. Each of these regulatory mechanism has varying effects on inflammation and vascular function regulation and work by distinct mechanism. Of these mechanisms the activation of the nuclear transcription factor PPAR-y is regarded as being beneficial. The study aimed to investigate the effect of the dietary lipid and polyunsaturated fatty acid CLA on the human monocytic cell line THP-I cells at physiological (20uM) and non physiological (100uM) concentrations. The effect of CLA on the secretion of TNF-a in pre-stimulated cell and post stimulated cell supernatants were collected from a previous flow cytometry investigation. Samples were assayed using ELISA technique and the data retrieved were analysed statistically, using ANOVA and 2-Sample t-test, which was performed which showed a significant difference between the means of TNF-α concentration from the pre and post conditions. The study showed that CLA was able to significantly reduce TNF-α secretion possibly via the proposed signal pathway of PPAR- y as shown by other studies. CLA and Rosiglitazone have shown to work through binding to PPAR-y. The mean TNF-α secreted in both pre and post CLA conditions were very much similar. For instance, 20uM CLA with IL-13 secreted on average 74% TNF-α and Rosiglitazone as compared to untreated cells and IL-l3 produced 71% of TNF-α on average in pre stimulated cells. This study would suggest that it is possible, that combining a diet rich in CLA, a PPAR-y agonist may be efficacious in the prevention in type 2 diabetes that may lead to atherosclerosis.
BSc (Hons) Biomedical Sciences