The effect of differing sources of fetal calf serum on the growth and proliferation of HEK293T cells
Cardiff Metropolitan University
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Fetal calf serum (FCS) is often used in cell culture to supply the cells with growth factors, hormones, attachment factors, nutrients and many other substances they require for growth and proliferation. However, variability between batches of serum can make it difficult to reproduce results obtained using cell culture. There can also be considerable difference in cost between different types of FCS depending on the manufacturer and country of origin. The aim of this investigation was therefore to compare cell proliferation between the higher cost Hyclone defined U.S. FCS and the less expensive Biosera South American FCS to determine if there was any difference in the cell proliferation achieved. For the comparison, HEK293T cells were cultured in media containing their respective FCS for a period of 4 days and an assay was carried out daily to determine the number of viable cells present. Viable cell number was determined by use of an XTT labelling reagent. Growth curves were then prepared for both sets of cells to enable proliferation to be compared. This investigation determined that cells cultured with Hyclone FCS showed proliferation up to 3 times greater than those in Biosera FCS. The higher cost Hyclone FCS was therefore superior to the Biosera FCS. It is thought that the maternal diet, the breed of cattle and methods used to remove contaminants are important factors influencing the constituents of FCS and therefore its performance in cell culture.
BSc (Hons) Applied Biomedical Science
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