Comparison of four chromogenic agar media for rapid identification of Methicillin-resistant Staphylococcus aureus (MRSA)
Barber, Carian Alexandra
Cardiff Metropolitan University
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The aim of this study is to determine if any of three commercially available media requiring 18-24 hours incubation (bioMérieux chromlD MRSA agar, Oxoid Brilliance™ MRSA agar and E&O CHROMagar MRSA agar) are as effective as the media currently used (bioMérieux chromlD MRSA) in the laboratory in which I work for the rapid detection of MRSA from clinical samples. Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium responsible for many difficult to treat infections due to its resistance to certain antibiotics. MRSA is especially problematic in hospital settings where many patients have open wounds, invasive devices or have weakened immune systems making them at greater risk from infection. It is important that the MRSA bacterium is detected as soon as possible so that treatment can begin. Therefore, most patients in hospital are swabbed in order to determine if the MRSA bacterium is present. Once the patient has been swabbed, the swabs are sent to a Microbiology laboratory for testing. The laboratory uses a solid growth media known as 'agar' to grow MRSA bacterium. The agar currently used in the laboratory requires 48 hours to grow MRSA. For this study 500 sets of MRSA screening swabs that have been sent to the microbiology laboratory for routine screening will be used. Four commercially available solid agar media will be used for the inoculation of each anonymous swab including; bioMérieux chromlD MRSA agar (requiring 24-48 hours incubation), a new chromlD MRSA agar from bioMérieux requiring 18-24 hours incubation, E&O Laboratories Ltd. Colorex MRSA with Cefoxitin and Oxoid Brilliance™ 2 MRSA agar. All plates will be observed four times (after 18, 20 ,24 and 48 hours incubation) for growth of MRSA colonies. All plates will be read by me only, and all results will be recorded. From the 1612 clinical samples used, 31 were confirmed as MRSA positive. All of the three commercially available media under test (new chromlD MRSA agar from bioMérieux, E&O Laboratories Ltd. Colorex MRSA with Cefoxitin and Oxoid Brilliance™ 2 MRSA agar) successfully isolated all positive MRSA samples within 24 hours, whereas the medium currently in use in the laboratory required 48 hours. From 1612 MRSA samples taken for clinical investigation, new chromlD MRSA agar from bioMérieux, E&O Laboratories Ltd. Colorex MRSA with Cefoxitin and Oxoid Brilliance™ 2 MRSA agar, were more efficient than chromlD MRSA agar from bioMérieux, for the isolation of MRSA from clinical samples.
BSc (Hons) Applied Biomedical Science
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