Manuka honey inhibits cell division in methicillin-resistant Staphylococcus aureus
Oxford University Press
MetadataDangos cofnod eitem llawn
OBJECTIVES: The aim of this study was to investigate the effect of manuka honey, artificial honey and an antibacterial component (methylglyoxal) on cell division in methicillin-resistant Staphylococcus aureus (MRSA). METHODS: Viability of epidemic MRSA-15 NCTC 13142 incubated with manuka honey, artificial honey and methylglyoxal was determined, and structural effects monitored by electron microscopy. Activity of murein hydrolase (a peptidoglycan-degrading enzyme implicated in cell separation, encoded by atl) was estimated by cell wall hydrolysis and zymography; expression of atl was quantified by real-time PCR. RESULTS: Growth of MRSA was inhibited by 5%, 10% and 20% (w/v) manuka honey and 10% (w/v) artificial honey containing methylglyoxal, but not 10% (w/v) artificial honey. Statistically significantly increased numbers of cells containing septa and increased cell diameter (P < 0.001 and P < 0.001, respectively) were found in MRSA exposed to 5%, 10% or 20% (w/v) manuka honey, but not 10% (w/v) artificial honey with and without methylglyoxal. Intracellular activity of murein hydrolase was elevated in MRSA grown in 10% (w/v) artificial honey and at undetectable levels in MRSA treated with 10% (w/v) manuka honey. Increased atl expression was found in MRSA treated with 10% (w/v) manuka honey and 10% artificial honey containing methylglyoxal. CONCLUSIONS: Enlarged cells containing septa were observed in MRSA exposed to inhibitory concentrations of manuka honey, suggesting that cell division was interrupted. These changes were not caused by either the sugars or methylglyoxal in honey and indicate the presence of additional antibacterial components in manuka honey.
Journal of Antimicrobial Chemotherapy;
Jenkins, R., Burton, N. and Cooper, R. (2011) 'Manuka honey inhibits cell division in methicillin-resistant Staphylococcus aureus', Journal of Antimicrobial Chemotherapy, 66(11), pp.2536-2542
Dynodwr Gwrthrych Digidol (DOI)http://dx.doi.org/10.1093/jac/dkr340
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Yn dangos eitemau sy’n perthyn drwy deitl, awdur, pwnc a chrynodeb.
Jenkins, Rowena (University of Wales, 2009)Preliminary studies have shown that manuka honey affects the cell cycle of MRSA by impeding cell division, but mode of action was unknown. Cell division depends on the formation of septa and cleavage of peptidoglycan at ...
Al-Maaini, Rahma Ali Saleh (University of Wales, 2012)Honey has been shown to have therapeutic properties, which include immunomodulatory and antibacterial activity in vitro and anti-inflammatory, antipyretic and wound healing properties in vivo. A complex mix of factors ...
Roberts, Aled Edward Lloyd; Brown, Helen Louise; Jenkins, Rowena (Dove press, 2015-10-29)Antimicrobial resistance (AMR) is an increasing clinical problem precipitated by the inappropriate use of antibiotics in the later parts of the 20th Century. This problem, coupled with the lack of novel therapeutics in ...