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dc.contributor.authorJenkins, Rowena
dc.contributor.authorBurton, Neil
dc.contributor.authorCooper, Rose
dc.date.accessioned2014-02-07T11:30:49Z
dc.date.available2014-02-07T11:30:49Z
dc.date.issued2011
dc.identifier.citationJenkins, R., Burton, N. and Cooper, R. (2011) 'Manuka honey inhibits cell division in methicillin-resistant Staphylococcus aureus', Journal of Antimicrobial Chemotherapy, 66(11), pp.2536-2542en_US
dc.identifier.issn0305-7453
dc.identifier.urihttp://hdl.handle.net/10369/5250
dc.description.abstractOBJECTIVES: The aim of this study was to investigate the effect of manuka honey, artificial honey and an antibacterial component (methylglyoxal) on cell division in methicillin-resistant Staphylococcus aureus (MRSA). METHODS: Viability of epidemic MRSA-15 NCTC 13142 incubated with manuka honey, artificial honey and methylglyoxal was determined, and structural effects monitored by electron microscopy. Activity of murein hydrolase (a peptidoglycan-degrading enzyme implicated in cell separation, encoded by atl) was estimated by cell wall hydrolysis and zymography; expression of atl was quantified by real-time PCR. RESULTS: Growth of MRSA was inhibited by 5%, 10% and 20% (w/v) manuka honey and 10% (w/v) artificial honey containing methylglyoxal, but not 10% (w/v) artificial honey. Statistically significantly increased numbers of cells containing septa and increased cell diameter (P < 0.001 and P < 0.001, respectively) were found in MRSA exposed to 5%, 10% or 20% (w/v) manuka honey, but not 10% (w/v) artificial honey with and without methylglyoxal. Intracellular activity of murein hydrolase was elevated in MRSA grown in 10% (w/v) artificial honey and at undetectable levels in MRSA treated with 10% (w/v) manuka honey. Increased atl expression was found in MRSA treated with 10% (w/v) manuka honey and 10% artificial honey containing methylglyoxal. CONCLUSIONS: Enlarged cells containing septa were observed in MRSA exposed to inhibitory concentrations of manuka honey, suggesting that cell division was interrupted. These changes were not caused by either the sugars or methylglyoxal in honey and indicate the presence of additional antibacterial components in manuka honey.en_US
dc.language.isoenen_US
dc.publisherOxford University Pressen_US
dc.relation.ispartofseriesJournal of Antimicrobial Chemotherapy;
dc.subjectcytokinesisen_US
dc.subjectcell cycleen_US
dc.subjectmurein hydrolaseen_US
dc.subjectMRSAen_US
dc.subjectmanuka honeyen_US
dc.titleManuka honey inhibits cell division in methicillin-resistant Staphylococcus aureusen_US
dc.typeArticleen_US
dc.identifier.doihttp://dx.doi.org/10.1093/jac/dkr340


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