Proteomic and genomic analysis of methicillin-resistant Staphylococcus aureus (MRSA) exposed to manuka honey in vitro demonstrated down-regulation of virulence markers
Author
Jenkins, Rowena
Burton, Neil
Cooper, Rose
Date
2013-10-31Type
Article
Publisher
Oxford University Press
ISSN
0305-7453
Metadata
Show full item recordAbstract
Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) isan important pathogen. Its resistance to multiple
antibiotics and its prevalence in healthcare establishments make it a serious threat to human health that requires
novel interventions. Manuka honey is a broad-spectrum antimicrobial agent that is gaining acceptance in the
topical treatment of wounds. Because its mode of action is only partially understood, proteomic and genomic
analysis was used to investigate the effects of manuka honey on MRSA at a molecular level.
Methods: Two-dimensional gel electrophoresis with dual-channel imaging was combined with matrix-assisted
laser desorption ionization–time of flight massspectrometry to determine the identities of differentiallyexpressed
proteins. The expression of the corresponding geneswas investigated by quantitative PCR. Microarray analysis provided
an overview of alterations in gene expression across the MRSA genome.
Results: Genes with increased expression following exposure to manuka honey were associated with glycolysis,
transport and biosynthesis of amino acids, proteins and purines. Those with decreased expression were involved
in the tricarboxylic acid cycle, cell division, quorum sensing and virulence. The greatest reductions were seen in
genes conferring virulence (sec3, fnb, hlgA, lip and hla) and coincided with a down-regulation of global regulators,
such as agr, sae and sarV. A model to illustrate these multiple effects was constructed and implicated glucose,
which is one of the major sugars contained in honey.
Conclusions: A decreased expression of virulence genes in MRSA will impact on its pathogenicity and needs to be
investigated in vivo.
Journal/conference proceeding
Journal of Antimicrobial Chemotherapy;
Citation
Jenkins, R., Burton, N. and Cooper, R. (2014) Proteomic and genomic analysis of methicillin-resistant Staphylococcus aureus (MRSA) exposed to manuka honey in vitro demonstrated down-regulation of virulence markers', Journal of Antimicrobial Chemotherapy, 69(3), pp.603-615.
Description
This article was published on 31 October 2013 (online), available open access at: https://doi.org/10.1093/jac/dkt430
Collections
- Un-themed [116]
Related items
Showing items related by title, author, subject and abstract.
-
Manuka honey is bactericidal against Pseudomonas aeruginosa and results in differential expression of oprF and algD
Roberts, Aled Edward Lloyd; Maddocks, Sarah; Cooper, Rose (Society for General Microbiology, 2012)The presence of Pseudomonas aeruginosa in cutaneous wounds is of clinical significance and can lead to persistent infections. Manuka honey has gained ground in clinical settings due to its effective therapeutic action ... -
The inhibition of Pseudomonas aeruginosa by manuka honey.
Roberts, Aled Edward Lloyd (Cardiff Metropolitan University, 2014)Although manuka honey has been shown to inhibit Pseudomonas aeruginosa, an opportunistic pathogen implicated in cutaneous wound infections, the mechanisms of action are not yet defined. The purpose of this study, therefore, ... -
Effect of manuka honey on the expression of universal stress protein A in meticillin-resistant Staphylococcus aureus
Jenkins, Rowena; Burton, Neil; Cooper, Rose (Elsevier, 2011)Staphylococcus aureus is an important pathogen that can cause many problems, from impetigo to endocarditis. With its continued resistance to multiple antibiotics, S. aureus remains a serious health threat. Honey has been ...