• English
    • Welsh
  • English 
    • English
    • Welsh
  • Login
Search DSpace:
  • Home
  • Research at Cardiff Met
  • Library Services
  • Contact Us
View item 
  • DSpace home
  • Cardiff School of Sport and Health Sciences
  • Cardiovascular Health and Ageing
  • View item
  • DSpace home
  • Cardiff School of Sport and Health Sciences
  • Cardiovascular Health and Ageing
  • View item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Protocols to detect senescence-associated beta-galactosidase (SA-βgal) activity, a biomarker of senescent cells in culture and in vivo

Thumbnail
Author
Debacq-Chainiaux, Florence
Erusalimsky, Jorge
Campisi, Judith
Toussaint, Olivier
Date
2009
Type
Article
Publisher
Nature Publishing Group
ISSN
1754-2189
Metadata
Show full item record
Abstract
Normal cells can permanently lose the ability to proliferate when challenged by potentially oncogenic stress, a process termed cellular senescence. Senescence-associated beta-galactosidase (SASA-βgal) activity, detectable at pH 6.0, permits the identification of senescent cells in culture and mammalian tissues. Here we describe first a cytochemical protocol suitable for the histochemical detection of individual senescent cells both in culture and tissue biopsies. The second method is based on the alkalinization of lysosomes, followed by the use of 5-dodecanoylaminofluorescein di-β-D-galactopyranoside (C12FDG), a fluorogenic substrate for βgal activity. The cytochemical method takes about 30 min to execute, and several hours to a day to develop and score. The fluorescence methods take between 4 and 8 h to execute and can be scored in a single day. The cytochemical method is applicable to tissue sections and requires simple reagents and equipment. The fluorescence-based methods have the advantages of being more quantitative and sensitive.
Journal/conference proceeding
Nature Protocols;
Citation
Nature Protocols 4 (12), pp. 1798 - 1806 (2009)
URI
http://hdl.handle.net/10369/5286
DOI
http://dx.doi.org/10.1038/nprot.2009.191
Collections
  • Cardiovascular Health and Ageing [154]

Related items

Showing items related by title, author, subject and abstract.

  • Thumbnail

    SIRT6 protects human endothelial cells from DNA damage, telomere dysfunction, and senescence 

    Cardus, Anna; Uryga, Anna; Walters, Gareth; Erusalimsky, Jorge (Oxford University Press, 2013)
    AIMS: Although endothelial cell senescence is known to play an important role in the development of cardiovascular pathologies, mechanisms that attenuate this process have not been extensively investigated. The aim of this ...
  • Thumbnail

    The angiotensin-(1-7)/Mas receptor a xis protects from endothelial cell senescence via klotho and Nrf2 activation 

    Romero, Alejandra; Hipólito-Luengo, Álvaro San; Villalobos, Laura; Vallejo, Susana; Valencia, Inés; Michalska, Patrycja; Pajuelo-Lozano, Natalia; Sánchez-Pérez, Isabel; León, Rafael; Bartha, José Luis; Sanz, María J.; Erusalimsky, Jorge; Sánchez-Ferrer, Carlos F.; Romacho, Tania; Peiró, Concepción (Wiley, 2019-02-17)
    Endothelial cell senescence is a hallmark of va scular aging that pre disposes to vascular disease. We aimed to explore the capacity of the renin-angiotensin system (RAS) heptapeptide angiotensin ...
  • Thumbnail

    Cellular senescence after single and repeated balloon catheter denudations of rabbit carotid arteries 

    Fenton, M.; Barker, S.; Kurz, D. J.; Erusalimsky, Jorge (American Heart Association, 2001)
    The hypothesis that increased cellular proliferation in the vasculature may lead to replicative senescence has been tested in a model of neointima formation. We have used a biomarker of replicative senescence, senescence ...

Browse

DSpace at Cardiff MetCommunities & CollectionsBy issue dateAuthorsTitlesSubjectsThis collectionBy issue dateAuthorsTitlesSubjects

My Account

Login

Statistics

Most Popular ItemsStatistics by CountryMost Popular Authors

DSpace software copyright © 2002-2015  DuraSpace
Contact us | Send feedback | Administrator