Protocols to detect senescence-associated beta-galactosidase (SA-βgal) activity, a biomarker of senescent cells in culture and in vivo
Nature Publishing Group
MetadataDangos cofnod eitem llawn
Normal cells can permanently lose the ability to proliferate when challenged by potentially oncogenic stress, a process termed cellular senescence. Senescence-associated beta-galactosidase (SASA-βgal) activity, detectable at pH 6.0, permits the identification of senescent cells in culture and mammalian tissues. Here we describe first a cytochemical protocol suitable for the histochemical detection of individual senescent cells both in culture and tissue biopsies. The second method is based on the alkalinization of lysosomes, followed by the use of 5-dodecanoylaminofluorescein di-β-D-galactopyranoside (C12FDG), a fluorogenic substrate for βgal activity. The cytochemical method takes about 30 min to execute, and several hours to a day to develop and score. The fluorescence methods take between 4 and 8 h to execute and can be scored in a single day. The cytochemical method is applicable to tissue sections and requires simple reagents and equipment. The fluorescence-based methods have the advantages of being more quantitative and sensitive.
Nature Protocols 4 (12), pp. 1798 - 1806 (2009)
Dynodwr Gwrthrych Digidol (DOI)http://dx.doi.org/10.1038/nprot.2009.191
Yn dangos eitemau sy’n perthyn drwy deitl, awdur, pwnc a chrynodeb.
Cardus, Anna; Uryga, Anna; Walters, Gareth; Erusalimsky, Jorge (Oxford University Press, 2013)AIMS: Although endothelial cell senescence is known to play an important role in the development of cardiovascular pathologies, mechanisms that attenuate this process have not been extensively investigated. The aim of this ...
The angiotensin-(1-7)/Mas receptor a xis protects from endothelial cell senescence via klotho and Nrf2 activation Romero, Alejandra; Hipólito-Luengo, Álvaro San; Villalobos, Laura; Vallejo, Susana; Valencia, Inés; Michalska, Patrycja; Pajuelo-Lozano, Natalia; Sánchez-Pérez, Isabel; León, Rafael; Bartha, José Luis; Sanz, María J.; Erusalimsky, Jorge; Sánchez-Ferrer, Carlos F.; Romacho, Tania; Peiró, Concepción (Wiley, 2019-02-17)Endothelial cell senescence is a hallmark of va scular aging that pre disposes to vascular disease. We aimed to explore the capacity of the renin-angiotensin system (RAS) heptapeptide angiotensin ...
Cellular senescence after single and repeated balloon catheter denudations of rabbit carotid arteries Fenton, M.; Barker, S.; Kurz, D. J.; Erusalimsky, Jorge (American Heart Association, 2001)The hypothesis that increased cellular proliferation in the vasculature may lead to replicative senescence has been tested in a model of neointima formation. We have used a biomarker of replicative senescence, senescence ...