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dc.date.accessioned2014-02-11T10:07:38Z
dc.date.available2014-02-11T10:07:38Z
dc.date.issued2010
dc.identifier.citationThe Journal of Lipid Research, 51, 334-344.en_US
dc.identifier.issn0022-2275
dc.identifier.urihttp://hdl.handle.net/10369/5288
dc.identifier.urihttp://dx.doi.org/10.1194/jlr.M000513
dc.descriptionThis research was originally published in Journal of Lipid Research. © the American Society for Biochemistry and Molecular Biologyen_US
dc.description.abstractIn addition to providing mechanical stability, growing evidence suggests that surfactant lipid components can modulate inflammatory responses in the lung. However, little is known of the molecular mechanisms involved in the immunomodulatory action of surfactant lipids. This study investigates the effect of the lipid-rich surfactant preparations Survanta®, Curosurf®, and the major surfactant phospholipid dipalmitoylphosphatidylcholine (DPPC) on interleukin-8 (IL-8) gene and protein expression in human A549 lung epithelial cells using immunoassay and PCR techniques. To examine potential mechanisms of the surfactant lipid effects, Toll-like receptor 4 (TLR4) expression was analyzed by flow cytometry, and membrane lipid raft domains were separated by density gradient ultracentrifugation and analyzed by immunoblotting with anti-TLR4 antibody. The lipid-rich surfactant preparations Survanta®, Curosurf®, and DPPC, at physiological concentrations, significantly downregulated lipopolysaccharide (LPS)-induced IL-8 expression in A549 cells both at the mRNA and protein levels. The surfactant preparations did not affect the cell surface expression of TLR4 or the binding of LPS to the cells. However, LPS treatment induced translocation of TLR4 into membrane lipid raft microdomains, and this translocation was inhibited by incubation of the cells with the surfactant lipid. This study provides important mechanistic details of the immune-modulating action of pulmonary surfactant lipids.en_US
dc.language.isoenen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biologyen_US
dc.relation.ispartofseriesJournal of Lipid Research;
dc.subjectlipopolysaccharideen_US
dc.subjectcytokinesen_US
dc.subjectinflammationen_US
dc.titleSurfactant lipids regulate LPS-induced interleukin-8 production in A549 lung epithelial cells by inhibiting translocation of TLR4 into lipid raft domainsen_US
dc.typeArticleen_US


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