The effect of the flavanol morin on biofilm formation, expression of fibronectin-binding proteins and aggregation properties of streptococcus pyogenes.
Green, Angharad Ellen
Cardiff Metropolitan University
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The aim of this study was to establish the impact of the flavanol Morin on S.pyogenes biofilm formation and expression of streptococcal fibronectin binding proteins. Whilst also investigating if the flavanol affects the aggregation properties of streptococci. Flavonols have proven in-vitro abilities to inhibit Gram positive sortase enzymes; consequently disrupting the anchoring of surface proteins to bacterial cell walls. Sortase inhibition results in bacteria with reduced capacity to adhere to fibronectin, therefore obstructing biofilm formation. The effect of Morin on S.pyogenes biofilm biomass was determined by comparing OD absorbances between control group bacteria, with those subjected incubated with flavanol and a significant difference was demonstrated (P <0.001). Experimental groups exhibited reduced OD readings indicating that Morin decreases biofilm formation. Flavanol concentrations of 227µM and 300µM displayed the biggest impact on biofilm biomass causing reductions of 64.28% and 64.77% respectively. RT-PCR demonstrated that Morin had no effect on Streptococcal Fibronectin binding protein-1 expression and it exhibited the ability to up-regulate expression of Serum Opacity Factor. Highlighting that Morin does not cause inhibit the S.pyogenes sortase enzyme; this flavanol must affect another essential mechanism which is involved in the formation of S.pyogenss biofilms. Flavanols induce rapid bacterial aggregation; large aggregate clumps of bacteria are able to decrease the microorganism's virulence. This study discovered that Morin causes S.pyogenes to form increased amounts of aggregates in a significantly shorter period of time when compared to those not incubated with the flavanol. The flavanol causes the bacteria to aggregate by more than 55% within 1 hour whereas those of the control group only demonstrate approximately 10% aggregation. The findings of this study highlight important characteristics of Morin to enable it to be an effective antimicrobial to combat S.pyogenes infections. The results provide an opportunity for further research to clarify mechanisms Morin uses to decrease biofilm biomass and to discover processes involved in its rapid bacterial aggregation abilities.
MSc Biomedical Science
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