Effect of manuka honey and its organic extract on production of GRO-alpha by human umbilical venous endothelial cell line
Cardiff Metropolitan University
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Honey has been utilized since ancient times for the management of different types of wounds. Only a few studies have been performed to explore which active components of Manuka honey cause the stimulation of production of cytokines and growth factors. According to recent studies done by Al-Amri (2009) and Lewis (2009), Manuka honey and its organic extract can stimulate an endothelial cell line to release chemokines such as IL-8 and GRO-α. The aim of the current study was to examine effect of both Manuka honey and its organic extract on production of GRO-α that might influence a mechanism of wound healing (angiogenesis), also to determine whether the component that causes stimulation of GRO-α production is present in Manuka honey or in its organic extract. HUVECs (a human umbilical venous endothelial cell line) were treated with 0.75% Manuka honey and 7.5% Manuka organic honey extract, without and without prior incubation with 10 µg/ml cycloheximide for 30 minutes. An Enzyme Linked Immunosorbent Assay (ELISA) was used to measure the concentration of GRO-α in supernatants of culture media (taken at time points up to 24 hours after stimulation). Results indicated that Manuka honey had slightly stimulatory effect on production of GRO-α, however the level of GRO-α after treatment with 7.5% organic Manuka honey extract showed a decrease. Furthermore, 10 µg/ml cycloheximide had no inhibitory effect on production of GRO-α, however, 10 µg/ml cycloheximide with 7.5% organic Manuka honey extract had extremely stimulatory effect (Up regulation) on the production of GRO-α particularly at 12 hours. According to previous studies done by other scientist suggested that elevated expression of GRO-α has been described in wound repair and angiogenesis, resulting in the role of GRO-α as potent chemoattraction for leukocytes, as well it is potent promoter of angiogenesis. Consequently, the critical role of chemokines as inflammatory cytokines has been reported, also positive and negative growth regulators, it seems conceivable that chemokines would be play on therapeutically as important adjuvant to encourage wound repair (angiogenesis). Overall, further studies are required to identify the components and mechanisms by which both Manuka honey and its organic extract effect on HUVEC to produce chemokines like IL-8 and GRO-α.
MSc Biomedical Sciences
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