AMPK agonists are exercise mimetics via PGC1alpha in monocytic cells
Al-Kalbani, Abir Mubarak Sulaiyam
Cardiff Metropolitan University
MetadataShow full item record
Introduction: There is evidence that acute bouts of exercise may enhance the immunity. AMPK activation by exercise has beneficial effects in many diseased groups such as diabetic and atherosclerosis. In this study our aim is to test the hypothesis that AMPK, when activated by exercise mimics (AICAR or Oligomycin), enters a complex with PGC1α, and possibly with PPARy, so changing gene expression in monocytes (THP-1 cell line). Methods: THP-1 cells were cultured to assess AMPK activity. These cells were treated with AICAR for 15 minutes at 37C. Total proteins extracts and nuclear extracts were obtained from control and AlCAR-treated cells. The effect of AICAR on AMPK phosphorylation was determined via Western blotting using anti-phospho-AMPK antibodies. Protein-protein interactions between AMPK and PGC1α were assessed via Co-ímmunoprecipitation/Western Blotting. Results: AMPK was phosphorylated in THP-1 cells by AICAR but AICAR treatment did not result in protein-protein interactions between AMPK and PGC1α. Conclusion: In a different cell-type (ie. skeletal muscle myocytes) the activation of AMPK has been shown to lead to phosphorylation of PGC1α, a reaction which leads to upregulation of many genes. However, in this study we suggest that AICAR is unable to phosphorylate PGC1α and thus affect expression of PGC1α-regulated genes in monocytes.
BSc (Hons) Biomedical Science
Showing items related by title, author, subject and abstract.
A study investigating sports students beliefs and their understanding about protein supplementation. Viljoen, Karlien (Cardiff Metropolitan University, 2011)Introduction: There is a lot of pressure among athletes to be the best and the strongest causing many to start using supplements. Protein supplements have become among the most popular types of supplements used by athletes. ...
Brown, Sophie (2018-04-01)Background: UK adult protein consumption far exceeds recommendations. Market research suggests a recent enthusiasm for high protein diets, the potential physiological effects of which have been extensively researched. Less ...
PRODUCTION AND RELEASE OF CXCL8/IL-8 AND TNF-α BY HaCaT KERATINOCYTES STIMULATED WITH ZIKA VIRUS ENVELOPE PROTEIN. Ogunleye, Simbiat (Cardiff Metropolitan University, 2017-06-01)Flaviviruses are associated with important human disease such as dengue fever, yellow fever and recently Zika infection. All this flaviviruses pose an ongoing public health threat as the number of outbreaks are increasing ...