The effect of different sources of media components on the growth and proliferation of HT1080 cell line
Al Balushi, Aliya
Cardiff Metropolitan University
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One of the important factors for optimizing the growth of cell culture in vitro is the selection of proper media. As there is inconsistency in different literatures available in the use of media used to grow HT1080 cell line, our aim of this study was to optimize the growth of HT1080 cell line using two different media. Human fibrosarcoma cell line HT1080 (ATCC-CCL121) were obtained and subcultured starting from 11 passage in to MEM media supplemented with 10% FBS and 1% glutamine incubated at 37oC with 5% CO2. To know at which concentration the log phase of HT1080 cells begin, XTT cell proliferation assay was performed. The HT1080 cells are then subcultured into two types of MEM media PAA and Invitrogen and supplemented as above. The cells were grown with the new media for one week to get adapted to it. Then a comparison in the growth of HT1080 cells with the two media is done by XTT proliferation assay and readings taken by Elisa reader at 450nm. The logarithmic phase of HT1080 cells started at concentration approximately 6000 cell/well (or 60,000 cells/ml). The growth curves shows a steady increase in the absorbance readings following the first day of cell seeding with both media. This indicates good growth of HT1080 cells with both media and there is almost a similar pattern of growth with both media. In conclusion, HT1080 cells can be successfully grown in both PAA and Invitrogen MEM media supplemented with 1% glutamine and 10% FBS. These data indicate that as long the media contain all the important components at right concentration and of good quality it should enhance the cell growth without any problem.
BSc (Hons) Biomedical Science
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