Examining the effects of iron concentration on the expression of Streptococcus pyogenes surface proteins
Cardiff Metropolitan University
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Introduction: Streptococcus pyogenes has established itself as a successful pathogen by the variety of virulence factors it possesses, including surface-bound adhesive proteins. This study aimed to detect the presence of four specific surface adhesin genes, and determine the effect of iron restriction on biofilm formation. Materials and Methods: Optimum growth conditions (i.e. Streptococcal concentration) were determined by growing S.pyogenes MGAS6180 at a variety of concentrations in the presence and absence of dipyrydyl solution, an iron chelator. Optimum iron concentration was determined in a similar way. Large scale biofilm production was optimised using a novel method, for successful RNA extraction. PCR reactions were used to detect the presence of the four streptococcal surface protein genes. Results: The optimum concentration of S. pyogenes was determined to be 3µl, and the optimum DIP concentration was 400µM. An increase in biomass was observed following treatment with DlP. The AspA, Sof, Spy0109 and Spy0632 genes were found to be present in the MGAS6180 strain. Discussion: The data obtained shows that at varying concentrations of iron chelation, biomass in S.pyogenes biofilms is increased. This suggests that it is likely that it also increases the expression of surface adhesin genes, although further work is needed to validate this.
BSc (Hons) Biomedical Science
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