The effect of cis-9, trans-11 conjugated linoleic acid isomer on CD36 expression in monocytic leukemia (THP-1) cells
Al-Shaqsi, Hanaa Salim Juma
Cardiff Metropolitan University
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Conjugated linoleic acid (CLA) refers to a group of naturally occurring conjugated dienoic isomers of linoleic acid (C18:2), of which the cis-9, trans-11 and trans-10, cis-12 isomers are predominate. It is attracting great interest among nutritionists and pathologists because it is a natural fat component that demonstrated to possess anticancer properties protecting several tissues from developing cancer. It appears Cis9, trans11 octa decadienoate is the common natural isomer found in milk and dairy products, where it has arisen as a by-product or intermediate in the microbial bio hydrogenation of linoleic acid in the rumen. Accumulating evidence has demonstrated that CLA isomers are capable of inhibiting the growth of a variety of cancer cell lines in vitro; however, their modulatory effects on the proliferation and differentiation of myeloid leukaemia cells remain poorly understood. CLA is a natural ligand for the most important nuclear transcription family which is the PPARs. Most notably of the PPAR-y subtype. PPARs are having important roles in development and maturation of the haematopoietic cells. CD36 is expressed by haematopoietic cells, including erythroid precursors, platelets, monocytes/macrophages...etc. CD36 is regulated by PPAR-y transcription factor. In the present study, we used monocytic cell line THP-1 to evaluate the role of cis9, trans11-CLA in modulating the expression of CD36 receptor. THP-1 cells were treated with cis-9, trans11-CLA alone and incubated for different time periods (8, 24,48 and 72 hours) respectively. Fluorescence-Activated Cell sorter (FACS) demonstrated CLA has modulated the expression of CD36 (P<0.05 ANOVA) and it reached the maximum expression after 72 hours. The second part of the experiment and this divided in to two parts. First part, THP-1 cells were treated with MK886, PPAR-α antagonistic prior to the addition of CLA and the cells were incubated for two different times (6 and 24 hours). FACS demonstrated that the expression of CD36 was inhibited which emphasizes the CD36 expression is modulated by PPAR-α. The second part of the experiment, THP-1 cells were treated for an hour with N- Carboxy Methyl Lysin (CML) then, MK886 prior to the stimulation of the cells with cis9, trans11-CLA. FACS demonstrated there were no significant changes in the modulation of the CD36 expression. In conclusion, the cis9 trans11-CLA up regulates the expression of CD36 through PPAR-α.
BSc (Hons) Biomedical Sciences
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