An investigation into the interaction of honey and oxacilin on methicillin-resistant staphylococcus aureus (MRSA)
Kazimoto, Theckla Selwyn
Cardiff Metropolitan University
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The emergence of multiple drug-resistant organisms involves opportunistic pathogens like MRSA, which have become a common cause of difficult-to-treat and life-threatening infections due to their resistance to most commonly-used antibiotics. MRSA has long been implicated in the spread of nosocomial infections, but now is posing a threat to healthy individuals, being linked with serious community-associated infections in different communities. The current prevalence of antibiotic-resistant microbial species has led to a re-evaluation of the therapeutic use of ancient remedies, including honey, which has evidence indicates is a reliable medicinal agent, being currently used in clinical treatment, especially of bacterial-infected wounds. Combination therapy is another approach which has been in practised for long time. This has been recognised as an important method offering effective short-term treatment and also preventing the development of resistance, as long as the combined agents give significant synergistic effects. The main aim of the present study was to investigate the effect of honey on susceptibility of MRSA to oxacillin. Three different methods were employed: E-test, agar incorporation test and chessboard titration, from which FIC was calculated. The E-test and agar incorporation test indicated that manuka honey can decrease MIC values of oxacillin, while chessboard titration confirmed a synergistic and additive effect with neither antagonism nor indifference interaction. The present investigation suggests that manuka honey can effectively be used in combination with B-lactams to potentiate their antibacterial activity. However, this study has provided a starting point for similar further studies aimed at finding the most effective treatment for infections caused by MRSA, to prevent their development into multi-drug resistance.
MSc Biomedical Science
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