The effect of Manuka Honey Organic extraxt on the production of growth factors/cytokines by human umbilical venous endothelial cell line
Al-Amri, Ahmed Hamed
Cardiff Metropolitan University
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Various anecdotal reports and case studies have already been published regarding the potential power of honey in the healing of chronic and non-healing types of wounds. Such reports have not referred to a particular component in honey that plays significant role in the enhancement of wound healing. The aim of this study was to investigate the possible effect of honey organic extract on the production of growth factors/cytokines, which might impact upon the wound healing process. Human umbilical venous endothelial cells (HUVEC) were treated with various concentrations of Manuka honey organic extract over a period of three days in the presence or absence of polymyxin B. Cells' growth rate was evaluated by CellTiter 96® AQueous One Solution Cell Proliferation Assay and IL-8 production was measured by subjecting supernatants of culture media to IL-8 enzyme linked immunosorbent assay (ELISA). Organic extract itself had no significant effect on HUVEC growth rate and IL-8 production. However, the cells were found to optimally proliferate and increase their production of IL-8 in the first 6 hours of incubation with 7.5% Manuka honey organic extract in the presence of 70 IU/ml polymyxin B.
MSc Biomedical Science
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