The effect of manuka honey organic extract on secretion of TNF-alpha alpha ans IL-8 from human monocytes
Cardiff Metropolitan University
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Since the times immemorial, honey has been used as a therapeutic agent to aid in wound healing. Honey possesses unknown substances that make it antibacterial and capable of stimulating the immune response. Though there is evidence suggesting the antibacterial effect of honey, there is little subjective evidence of honey stimulating wound healing. Honey is found to stimulate inflammatory cytokines like tumor necrosis factor alpha (TNF-α), IL-1β, interleukin-6 (IL-6). Honey was also found to reduce the ROI (reactive oxygen intermediates)-these findings show the important role of honey in wound healing, but the mechanism by which honey stimulates the wound healing is not fully understood. It is also not clearly evident as to which component of the honey is stimulating the process of wound healing. This study was performed to investigate and to determine the role of manuka honey organic extract in stimulating human monocytes (Mono Mac 6 cells) to secrete important inflammatory cytokines like TNF-α and another chemokine interleukin-8 (IL-8) which plays a very important role in wound healing. The Mono Mac 6 cells (MM6) were incubated with 1% (w/v) of manuka honey organic extract and the supernatants were tested using enzyme linked immunosorbent assay (ELISA) for TNF-α and interlukin-8 (IL-8) secretion. Incubation of MM6 cells with manuka honey organic extract has significantly increased the IL-8 secretion and there was no TNF-α secretion observed.
MSc Biomedical Sciences (Medical Microbiology)
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