Manuka honey inhibits the development of Streptococcus pyogenes biofilms and causes reduced expression of two fibronectin binding proteins
Society for General Microbiology
MetadataShow full item record
Streptococcus pyogenes (group A Streptococcus; GAS) is always of clinical significance in wounds where it can initiate infection, destroy skin grafts and persist as a biofilm. Manuka honey has broad spectrum antimicrobial activity and its use in the clinical setting is beginning to gain acceptance with the continuing emergence of antibiotic resistance and the inadequacy of established systemic therapies; novel inhibitors may affect clinical practice. In this study, the effect of manuka honey on S. pyogenes (M28) was investigated in vitro with planktonic and biofilm cultures using MIC, MBC, microscopy and aggregation efficiency. Bactericidal effects were found in both planktonic cultures and biofilms, although higher concentrations of manuka honey were needed to inhibit biofilms. Abrogation of adherence and intercellular aggregation was observed. Manuka honey permeated 24 h established biofilms of S. pyogenes, resulting in significant cell death and dissociation of cells from the biofilm. Sublethal concentrations of manuka honey effectively prevented the binding of S. pyogenes to the human tissue protein fibronectin, but did not inhibit binding to fibrinogen. The observed inhibition of fibronectin binding was confirmed by a reduction in the expression of genes encoding two major fibronectin-binding streptococcal surface proteins, Sof and SfbI. These findings indicate that manuka honey has potential in the topical treatment of wounds containing S. pyogenes.
Microbiology (2012), 158, 781–790
This article has been published in final form at http://dx.doi.org/10.1099/mic.0.053959-0
- Un-themed 
Showing items related by title, author, subject and abstract.
The effect of manuka honey and the iron chelator 2, 2-dipyridyl on biofilm biomass of S. aureus, P. aeruginosa, and S. pyogenes Alnamani, Fatma (University of Wales Institute Cardiff, 2012)Bacterial biofilms are the main causes of infection in many cases of sever non healing wound infections. They form this community as a protective way to survive when there are not enough nutrients, and pH and temperature ...
Manuka honey treatment of biofilms of Pseudomonas aeruginosa results in the emergence of isolates with increased honey resistance Camplin, A. L.; Maddocks, Sarah (BioMed Central, 2014)Background Medical grade manuka honeys are well known to be efficacious against Pseudomonas aeruginosa being bactericidal and inhibiting the development of biofilms; moreover manuka honey effectively kills P. aeruginosa ...
The role of human tissue proteins in mediating aggregation and biofilm development in Streptococcus spp. Al Shamsi, Iman Khamis Mohammed (University of Wales Institute Cardiff, 2012)Streptococcus pyogenes (group A Streptococcus; GAS) is always of clinical significance in wounds where it can initiate infection. Streptococcus gordonii (part of mitis group of streptococci) is a normal flora of human oral ...