Manuka honey inhibits the development of Streptococcus pyogenes biofilms and causes reduced expression of two fibronectin binding proteins
Awdur
Lopez, Marta
Rowlands, Richard
Cooper, Rose
Maddocks, Sarah
Dyddiad
2012Math
Article
Cyhoeddwr
Society for General Microbiology
ISSN
1350-0872
Metadata
Dangos cofnod eitem llawnCrynodeb
Streptococcus pyogenes (group A Streptococcus; GAS) is always of clinical significance in
wounds where it can initiate infection, destroy skin grafts and persist as a biofilm. Manuka honey
has broad spectrum antimicrobial activity and its use in the clinical setting is beginning to gain
acceptance with the continuing emergence of antibiotic resistance and the inadequacy of
established systemic therapies; novel inhibitors may affect clinical practice. In this study, the effect
of manuka honey on S. pyogenes (M28) was investigated in vitro with planktonic and biofilm
cultures using MIC, MBC, microscopy and aggregation efficiency. Bactericidal effects were found
in both planktonic cultures and biofilms, although higher concentrations of manuka honey were
needed to inhibit biofilms. Abrogation of adherence and intercellular aggregation was observed.
Manuka honey permeated 24 h established biofilms of S. pyogenes, resulting in significant cell
death and dissociation of cells from the biofilm. Sublethal concentrations of manuka honey
effectively prevented the binding of S. pyogenes to the human tissue protein fibronectin, but did
not inhibit binding to fibrinogen. The observed inhibition of fibronectin binding was confirmed by a
reduction in the expression of genes encoding two major fibronectin-binding streptococcal
surface proteins, Sof and SfbI. These findings indicate that manuka honey has potential in the
topical treatment of wounds containing S. pyogenes.
Cyfnodolyn/trafodion cynhadledd
Microbiology;
Dyfyniad
Microbiology (2012), 158, 781–790
Disgrifiad
This article has been published in final form at http://dx.doi.org/10.1099/mic.0.053959-0
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