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dc.contributor.authorLopez, Marta
dc.contributor.authorRowlands, Richard
dc.contributor.authorCooper, Rose
dc.contributor.authorMaddocks, Sarah
dc.date.accessioned2014-03-11T16:42:38Z
dc.date.available2014-03-11T16:42:38Z
dc.date.issued2012
dc.identifier.citationMicrobiology (2012), 158, 781–790en_US
dc.identifier.issn1350-0872
dc.identifier.urihttp://hdl.handle.net/10369/5423
dc.identifier.urihttp://dx.doi.org/10.1099/mic.0.053959-0en_GB
dc.descriptionThis article has been published in final form at http://dx.doi.org/10.1099/mic.0.053959-0en_GB
dc.description.abstractStreptococcus pyogenes (group A Streptococcus; GAS) is always of clinical significance in wounds where it can initiate infection, destroy skin grafts and persist as a biofilm. Manuka honey has broad spectrum antimicrobial activity and its use in the clinical setting is beginning to gain acceptance with the continuing emergence of antibiotic resistance and the inadequacy of established systemic therapies; novel inhibitors may affect clinical practice. In this study, the effect of manuka honey on S. pyogenes (M28) was investigated in vitro with planktonic and biofilm cultures using MIC, MBC, microscopy and aggregation efficiency. Bactericidal effects were found in both planktonic cultures and biofilms, although higher concentrations of manuka honey were needed to inhibit biofilms. Abrogation of adherence and intercellular aggregation was observed. Manuka honey permeated 24 h established biofilms of S. pyogenes, resulting in significant cell death and dissociation of cells from the biofilm. Sublethal concentrations of manuka honey effectively prevented the binding of S. pyogenes to the human tissue protein fibronectin, but did not inhibit binding to fibrinogen. The observed inhibition of fibronectin binding was confirmed by a reduction in the expression of genes encoding two major fibronectin-binding streptococcal surface proteins, Sof and SfbI. These findings indicate that manuka honey has potential in the topical treatment of wounds containing S. pyogenes.en_US
dc.language.isoenen_US
dc.publisherSociety for General Microbiologyen_US
dc.relation.ispartofseriesMicrobiology;
dc.titleManuka honey inhibits the development of Streptococcus pyogenes biofilms and causes reduced expression of two fibronectin binding proteinsen_US
dc.typeArticleen_US


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