Molecular typing of clostridium septicum
Ticha, Ambi Alfred
Cardiff Metropolitan University
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Three PCR amplification-typing methods were evaluated for typing 50 isolates of Clostridium septicum, with the objectives of investigating the existence of genotypes within the species and establishing a typing method for this organism. Enterobacterial repetitive consensus (ERIC), repetitive extragenic palindromic units (REP) and the BOX elements were independently studied. This study revealed that C. septicum could be divided into at least 6 different genotypes by ERIC rep-PCR fingerprinting, which proved to be more discriminatory than REP and BOX PCR typing. Analysis of ERIC types of C. septicum revealed no correlation with their clinical sources but future application of this method may yield valuable epidemiological information.
MSc Biomedical Sciences