The incidences of antibiotic associated diarrhoea caused by clostridium perfringens at Llandough, University Hospital of Wales and associated local hospitals
Cardiff Metropolitan University
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Clostridium perfringens, a relatively large (5 x 1µm), gram positive, non-motile, capsulate, spore forming bacillus, and is well known as the cause of many diseases, such as gas gangrene, food poisoning and antibiotic associated diarrhoea (AAD). AAD caused by C.perfringens is thought to be on the increase, and it may be responsible for 5-20% of all AAD and sporadic cases of non-food-borne cases of diarrhoea. The aim of this study was to determine the incidence of AAD caused by C.perfringens in the in-patient populations of local hospitals, and determine if routine testing for C.perfringens was necessary. This was achieved by using a number of tests to isolate and identify C.perfringens, as well as to determine the relatedness of any isolates by Rep-PCR. The ELISA used was a quick and easy method to identify C.perfringens enterotoxin CpEnt, but gave a high number of false-positives. The use of an ELISA reader would have prevented the optimistic translation of weak ELISA results. Results obtained by this study suggest that routine C.perfringens testing may not be necessary as the incidence (0.96%) of C.perfringens AAD is low.
MSc Biomedical Sciences