Manuka honey is bactericidal against Pseudomonas aeruginosa and results in differential expression of oprF and algD
Author
Roberts, Aled Edward Lloyd
Maddocks, Sarah
Cooper, Rose
Date
2012Type
Article
Publisher
Society for General Microbiology
ISSN
1350-0872
Metadata
Show full item recordAbstract
The presence of Pseudomonas aeruginosa in cutaneous wounds is of clinical significance and
can lead to persistent infections. Manuka honey has gained ground in clinical settings due to its
effective therapeutic action and broad spectrum of antibacterial activity. In this study, the effect of
manuka honey on P. aeruginosa was investigated using MIC, MBC, growth kinetics, confocal
microscopy, atomic force microscopy and real-time PCR. A bactericidal mode of action for
manuka honey against P. aeruginosa was deduced (12 %, w/v, MIC; 16 %, w/v, MBC) and
confirmed by confocal and atomic force microscopy, which showed extensive cell lysis after
60 min exposure to inhibitory concentrations of manuka honey. The inability of honey-treated cells
to form microcolonies was demonstrated and investigated using Q-PCR for three key
microcolony-forming genes: algD, lasR and oprF. The expression of algD increased 16-fold
whereas oprF expression decreased 10-fold following honey treatment; lasR expression remained
unaltered. These findings confirm that manuka honey is effective at inducing cell lysis and identify
two targets, at the genetic level, that might be involved in this process.
Journal/conference proceeding
Microbiology;
Citation
Microbiology (2012), 158, 3005–3013
Description
This article has been published in final form at http://dx.doi.org/10.1099/mic.0.062794-0
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