Immunoregulation in large and small airway by surfactant phospholipid
Njoh, Anjang Bean
Cardiff Metropolitan University
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This study was conducted to establish whether expression of the mucin gene MUC 2 could be up regulated by the inflammatory cytokines tumour necrosis factor-α, interleukin-1, or lipopolysaccharide. Additionally the study hoped to determine whether the surfactant phospholipid, dipalmitoylphosphatidylcholine (DPPC), has a regulatory effect on the up-regulation of this mucin gene. Pulmonary surfactant is a unique mixture of lipids and surfactant-specific proteins of which 90% are lipids and DPPC is the most prominent component accounting for some 65% of total lipid. This surfactant covers the entire airway surface. Surfactant is not restricted to the alveolar compartment; it also reaches terminal conducting airways and is present in upper airway secretions. While the role of surfactant in alveolar compartment has been intensively elucidated both in health and disease states, the possible role of surfactant in the airways requires further research. The human alveolar epithelial cell line A549 and Calu3 were exposed to surfactant phospholipid (DPPC) and stimulated by pro-inflammatory cytokines and LPS. Changes in MUC2 gene expression and signalling responses were determined by RT-PCR. Cells stimulated by pro-inflammatory cytokines and LPS showed marked up-regulation of expression of the MUC2 gene. This up-regulation was markedly reduced in cells pre-incubated with DPPC. These results suggest a role for DPPC in the regulation of mucin production in the large airways.
MSc Biomedical Sciences
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