DPPC regulates COX-2 expression in monocytes via phosphorylation of CREB
Author
Morris, Keith
Tonks, Amanda J.
Jones, Ken
Ahluwalia, Maninder
Thomas, Andrew
Tonks, Alex
Jackson, S.K.
Date
2008Type
Article
Publisher
Elsevier
ISSN
0006-291X
Metadata
Show full item recordAbstract
The major phospholipid in pulmonary surfactant dipalmitoyl phosphatidylcholine (DPPC) has been shown to modulate inflammatory responses. Using human monocytes, this study demonstrates that DPPC significantly increased PGE2 (P < 0.05) production by 2.5-fold when compared to untreated monocyte controls. Mechanistically, this effect was concomitant with an increase in COX-2 expression which was abrogated in the presence of a COX-2 inhibitor. The regulation of COX-2 expression was independent of NF-κB activity. Further, DPPC increased the phosphorylation of the cyclic AMP response element binding protein (CREB; an important nuclear transcription factor important in regulating COX-2 expression). In addition, we also show that changing the fatty acid groups of PC (e.g. using l-α-phosphatidylcholine β-arachidonoyl-γ-palmitoyl (PAPC)) has a profound effect on the regulation of COX-2 expression and CREB activation. This study provides new evidence for the anti-inflammatory activity of DPPC and that this activity is at least in part mediated via CREB activation of COX-2
Journal/conference proceeding
Biochemical and Biophysical Research Communications
Citation
Morris, R.H.K., Tonks, A.J., Jones, K.P., Ahluwalia, M.K., Thomas, A.W., Tonks, A. and Jackson, S.K. (2008) 'DPPC regulates COX-2 expression in monocytes via phosphorylation of CREB', Biochemical and Biophysical Research Communications, 370(1), pp.174-178.
Description
The final published version is available at: http://dx.doi.org/10.1016/j.bbrc.2008.03.052
Collections
- Un-themed [116]
Related items
Showing items related by title, author, subject and abstract.
-
The effects of peroxisome proliferator-activated receptor-gamma (ppar-γ) agonists on monocytic cell activation and endothelial function in diabetes
Ahluwalia, Maninder (Cardiff Metropolitan University, 2005)Peroxisome Proliferator-activated Receptor-y (PPARy) is a ligand-activated transcription factor responsible for controlling genes involved in lipid and glucose metabolism. The thiazolidinediones (TZDs) are a class of ... -
Membrane and soluble CD14 expression in response to lipopolysaccharides exposure in monocytic cell models
Kwok, Eugene (Cardiff Metropolitan University, 2011)Background - CD14 is an important cell surface molecule of monocytic cells. It is involved in cellular activation in binding with bacterial endotoxin lipopolysaccharide (LPS). The receptor for lipopolysaccharide LPS (CD14) ... -
Peripheral cytokine expression in autoimmune thyroiditis: Effects of in vitro modulation by rosiglitazone and dexamethasone
Okosieme, O.E.; Parkes, A.B.; Premawardhana, L.D.K.E.; Thomas, Andrew; Evans, L.M.; Lazarus, J.H. (Mary Ann Liebert Inc., 2006-10-01)Background: In Hashimoto's thyroiditis (HT), there is evidence for activation of peripheral T-lymphocytes that predominantly express a T helper 1 (TH1) cytokine bias. However, the immunomodulatory factors involved in ...