DPPC regulates COX-2 expression in monocytes via phosphorylation of CREB
Tonks, Amanda J.
MetadataShow full item record
The major phospholipid in pulmonary surfactant dipalmitoyl phosphatidylcholine (DPPC) has been shown to modulate inflammatory responses. Using human monocytes, this study demonstrates that DPPC significantly increased PGE2 (P < 0.05) production by 2.5-fold when compared to untreated monocyte controls. Mechanistically, this effect was concomitant with an increase in COX-2 expression which was abrogated in the presence of a COX-2 inhibitor. The regulation of COX-2 expression was independent of NF-κB activity. Further, DPPC increased the phosphorylation of the cyclic AMP response element binding protein (CREB; an important nuclear transcription factor important in regulating COX-2 expression). In addition, we also show that changing the fatty acid groups of PC (e.g. using l-α-phosphatidylcholine β-arachidonoyl-γ-palmitoyl (PAPC)) has a profound effect on the regulation of COX-2 expression and CREB activation. This study provides new evidence for the anti-inflammatory activity of DPPC and that this activity is at least in part mediated via CREB activation of COX-2
Biochemical and Biophysical Research Communications
Morris, R.H.K., Tonks, A.J., Jones, K.P., Ahluwalia, M.K., Thomas, A.W., Tonks, A. and Jackson, S.K. (2008) 'DPPC regulates COX-2 expression in monocytes via phosphorylation of CREB', Biochemical and Biophysical Research Communications, 370(1), pp.174-178.
The final published version is available at: http://dx.doi.org/10.1016/j.bbrc.2008.03.052
- Un-themed 
Showing items related by title, author, subject and abstract.
The effects of peroxisome proliferator-activated receptor-gamma (ppar-γ) agonists on monocytic cell activation and endothelial function in diabetes Ahluwalia, Maninder (Cardiff Metropolitan University, 2005)Peroxisome Proliferator-activated Receptor-y (PPARy) is a ligand-activated transcription factor responsible for controlling genes involved in lipid and glucose metabolism. The thiazolidinediones (TZDs) are a class of ...
Membrane and soluble CD14 expression in response to lipopolysaccharides exposure in monocytic cell models Kwok, Eugene (Cardiff Metropolitan University, 2011)Background - CD14 is an important cell surface molecule of monocytic cells. It is involved in cellular activation in binding with bacterial endotoxin lipopolysaccharide (LPS). The receptor for lipopolysaccharide LPS (CD14) ...
Peripheral cytokine expression in autoimmune thyroiditis: Effects of in vitro modulation by rosiglitazone and dexamethasone Okosieme, O.E.; Parkes, A.B.; Premawardhana, L.D.K.E.; Thomas, Andrew; Evans, L.M.; Lazarus, J.H. (Mary Ann Liebert Inc., 2006-10-01)Background: In Hashimoto's thyroiditis (HT), there is evidence for activation of peripheral T-lymphocytes that predominantly express a T helper 1 (TH1) cytokine bias. However, the immunomodulatory factors involved in ...