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DPPC regulates COX-2 expression in monocytes via phosphorylation of CREB

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Author
Morris, Keith
Tonks, Amanda J.
Jones, Ken
Ahluwalia, Maninder
Thomas, Andrew
Tonks, Alex
Jackson, S.K.
Date
2008
Type
Article
Publisher
Elsevier
ISSN
0006-291X
Metadata
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Abstract
The major phospholipid in pulmonary surfactant dipalmitoyl phosphatidylcholine (DPPC) has been shown to modulate inflammatory responses. Using human monocytes, this study demonstrates that DPPC significantly increased PGE2 (P < 0.05) production by 2.5-fold when compared to untreated monocyte controls. Mechanistically, this effect was concomitant with an increase in COX-2 expression which was abrogated in the presence of a COX-2 inhibitor. The regulation of COX-2 expression was independent of NF-κB activity. Further, DPPC increased the phosphorylation of the cyclic AMP response element binding protein (CREB; an important nuclear transcription factor important in regulating COX-2 expression). In addition, we also show that changing the fatty acid groups of PC (e.g. using l-α-phosphatidylcholine β-arachidonoyl-γ-palmitoyl (PAPC)) has a profound effect on the regulation of COX-2 expression and CREB activation. This study provides new evidence for the anti-inflammatory activity of DPPC and that this activity is at least in part mediated via CREB activation of COX-2
Journal/conference proceeding
Biochemical and Biophysical Research Communications
Citation
Morris, R.H.K., Tonks, A.J., Jones, K.P., Ahluwalia, M.K., Thomas, A.W., Tonks, A. and Jackson, S.K. (2008) 'DPPC regulates COX-2 expression in monocytes via phosphorylation of CREB', Biochemical and Biophysical Research Communications, 370(1), pp.174-178.
URI
http://hdl.handle.net/10369/5453
http://dx.doi.org/10.1016/j.bbrc.2008.03.052
Description
The final published version is available at: http://dx.doi.org/10.1016/j.bbrc.2008.03.052
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