Comparison of solid and solvent phase extraction of caffeine from bovine serum albumin, using high pressure liquid chromatography for extract analysis
Cardiff Metropolitan University
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Since the early discovery and classification of caffeine, it has been used widely to develop extraction procedures performed on serum samples. Albumin; the most abundant plasma protein, has been shown to bind drugs using various sites and domains . Research into extraction from albumin has mostly relied on solvent usage; which is detrimental to the environment, also posing a threat to the researchers' health. The experiment was conducted to show possible alternatives using solid phase extraction instead of solvent, and also to assess the efficiency of current solvent methods. The results showed a steady declining trend on return (%) when using solvent extraction, thought to be due to increase in caffeine concentration. While this decrease in return ranged from 5-20%, the experiment still yielded consistent results on each performance. The SPE results were overwhelmed with protein interference, which has led to a detailed discussion of pre-treatment.
BSc (Hons) Biomedical Science
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