The antibacterial activity of selected honeys against Staphylococcus aureus
Cardiff Metropolitan University
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Honey has been used since the ancient times as a naturally remedy to treat wounds and be an antiseptic. It has been recorded in ancient hieroglyphics that selected honeys have been used for different purposes. In this study 21 honey samples from North Wales, Cornwall, and London were collected and tested for their antibacterial activity against staphylococcus aureus. A series of bio assay plates were prepared and seeded with the bacteria and the ability of the honey sample to inhibit the growth of the bacteria the antibacterial activity was calculated relevant to known phenol standards, the pH, water and sugar percentage concentration were also carried on samples A- E all other samples were not tested as there was an insufficient amount. The results showed that 5 of the honey samples did not present any antibacterial activity or were <2% w/v phenol equivalent. However the other 16 samples showed a good range of total activity ranging from 6% w/v phenol equivalent to 52% w/v equivalent. Samples that showed highest total antibacterial activity were from North Wales the floral source of the highest total antibacterial activity was Indian Balsam this particular sample of honey also present non peroxide activity this is unique finding as many of the British samples that have been tested have shown no non peroxide activity. The samples that presented antibacterial activity relevant to phenol have potential to be used as antiseptics but the potential for wound care use need to be studied. Sample C from North Wales needs to be studied further because of its high non peroxide activity if this high activity is correct then this sample has great potential for modern wound care management.
BSc (Hons) Bimedical Science
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